Sox10--a marker for not only schwannian and melanocytic neoplasms but also myoepithelial cell tumors of soft tissue: a systematic analysis of 5134 tumors.
Sox10--a marker for not only schwannian and melanocytic neoplasms but also myoepithelial cell tumors of soft tissue: a systematic analysis of 5134 tumors.
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DOI：
10.1097/pas.0000000000000398
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Fetsch JF
中科院分区：
文献类型：
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作者：
Miettinen M;McCue PA;Sarlomo-Rikala M;Biernat W;Czapiewski P;Kopczynski J;Thompson LD;Lasota J;Wang Z;Fetsch JF
Sox 10转录因子在雪旺氏细胞和黑素细胞谱系中表达，在它们的发育中很重要，可用作相应肿瘤的标记物。此外，在肌上皮/基底细胞上皮肿瘤的亚群中也有报道，但其表达仍不完全。在这项研究中，我们检测了Sox 10在5134例人类肿瘤中的表达，这些肿瘤包括神经外胚层、间叶、淋巴和上皮肿瘤。一种新的兔单克隆抗体（克隆EP 268）和Leica Bond Max自动化用于多肿瘤块库，每个载玻片包含30-70个病例。Sox 10在软组织和胃肠道的良性雪旺细胞瘤和转移性黑色素瘤中一致表达，并且在恶性外周神经鞘瘤中不存在。相反，Sox 10在许多潜在的神经鞘肿瘤的模拟物中是不存在的，例如细胞性神经鞘瘤、脑膜瘤、胃肠道间质瘤、PEComa和各种成纤维细胞-肌纤维母细胞肿瘤。Sox 10在间充质肿瘤中几乎不存在，但偶尔在腺泡状横纹肌肉瘤中可见。在软组织的上皮性肿瘤中，Sox 10仅在肌上皮瘤中表达，尽管在恶性变体中通常不表达。除了基底细胞型乳腺癌以外的癌，只有很少的阳性，但包括罕见的头颈部鳞状细胞癌和肺小细胞癌。此外，Sox 10往往局灶性表达，反映了原始Sox 10阳性表型或神经外胚层分化的胚胎癌。在诊断Sox 10阳性肿瘤时，必须考虑在各种肿瘤中包埋的非肿瘤性雪旺细胞或黑素细胞中Sox 10的表达。Sox 10抗体属于用于诊断软组织和上皮肿瘤的现代免疫组化组。
Sox10 transcription factor is expressed in Schwannian and melanocytic lineages and is important in their development and can be used as a marker for corresponding tumors. Additionally, it has been reported in subsets of myoepithelial/basal cell epithelial neoplasms, but its expression remains incompletely characterized. In this study, we examined Sox10 express-ion in 5134 human neoplasms spanning a wide spectrum of neuroectodermal, mesenchymal, lymphoid, and epithelial tumors. A new rabbit monoclonal antibody (clone EP268) and Leica Bond Max automation were used on multitumor block libraries containing 30–70 cases per slide. Sox10 was consistently expressed in benign Schwann cell tumors of soft tissue and the GI-tract and metastatic melanoma, and was variably present in malignant peripheral nerve sheath tumors. In contrast, Sox10 was absent in many potential mimics of nerve sheath tumors such as cellular neurothekeoma, meningioma, gastrointestinal stromal tumors, PEComa, and a variety of fibroblastic-myofibroblastic tumors. Sox10 was virtually absent in mesenchymal tumors but occasionally seen in alveolar rhabdomyosarcoma. In epithelial tumors of soft tissue, Sox10 was expressed only in myoepitheliomas, although often absent in malignant variants. Carcinomas, other than basal cell type breast cancers, were only rarely positive but included rare squamous carcinomas of head and neck and pulmonary small cell carcinomas. Furthermore, Sox10 was often focally expressed in embryonal carcinoma reflecting a primitive Sox10-positive phenotype or neuroectodermal differentiation. Expression of Sox10 in entrapped non-neoplastic Schwann cells or melanocytes in various neoplasms has to be considered in diagnosing Sox10-positive tumors. The Sox10 antibody belongs in a modern immunohistochemical panel for the diagnosis of soft tissue and epithelial tumors.