βC1, the pathogenicity factor of TYLCCNV, interacts with AS1 to alter leaf development and suppress selective jasmonic acid responses
βC1, the pathogenicity factor of TYLCCNV, interacts with AS1 to alter leaf development and suppress selective jasmonic acid responses
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DOI：
10.1101/gad.1682208
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发表时间：
2008-09-15
影响因子：
10.5
通讯作者：
Chua, Nam-Hai
中科院分区：
文献类型：
--
作者：
Yang, Jun-Yi;Iwasaki, Mayumi;Chua, Nam-Hai
病毒在植物上引起致病症状，但其分子基础尚不清楚。在这里，我们证明了表达番茄黄化曲叶中国病毒致病蛋白β1的转基因拟南芥能够在很大程度上复制感染病毒的烟草的症状，表现为叶片向上卷曲，叶片辐射状，远轴表面长出组织，以及不育花。在这些形态变化的同时，miR165/166水平降低，PHB和PHV转录水平上升。不对称叶1(AS1)和不对称叶2(AS2)作为AS1/AS2复合体调节叶片发育。值得注意的是，β-C_1在形态和分子水平上表现为高表达AS2的植物，并且β-C_1能够部分补充AS2突变。β1直接与AS1结合，引起依赖AS1而不依赖AS2的形态和基因表达变化，并减弱选择性茉莉酸(JA)反应基因的表达。我们的结果表明，βC1与AS1形成一个复合体，以执行其致病功能并抑制JA反应的一个子集。
Viruses induce pathogenic symptoms on plants but the molecular basis is poorly understood. Here, we show that transgenic Arabidopsis expressing the pathogenesis protein beta C1 of Tomato yellow leaf curl China virus (TYLCCNV), a geminivirus, can phenocopy to a large extent disease symptoms of virus-infected tobacco plants in having upward curled leaves, radialized leaves with outgrowth tissues from abaxial surfaces, and sterile flowers. These morphological changes are paralleled by a reduction in miR165/166 levels and an increase in PHB and PHV transcript levels. Two factors, ASYMMETRIC LEAVES 1 (AS1) and ASYMMETRIC LEAVES 2 (AS2), are known to regulate leaf development as AS1/AS2 complex. Strikingly, beta C1 plants phenocopy plants overexpressing AS2 at the morphological and molecular level and beta C1 is able to partially complement as2 mutation. beta C1 binds directly to AS1, elicits morphological and gene expression changes dependent on AS1 but not AS2, and attenuates expression of selective jasmonic acid (JA)-responsive gene. Our results show that beta C1 forms a complex with AS1 to execute its pathogenic functions and to suppress a subset of JA responses.