PRODUCTION AND MANIPULATION OF INDIVIDUAL MICROSCLEROTIA OF VERTICILLIUM-DAHLIAE FOR USE IN STUDIES OF SURVIVAL
PRODUCTION AND MANIPULATION OF INDIVIDUAL MICROSCLEROTIA OF VERTICILLIUM-DAHLIAE FOR USE IN STUDIES OF SURVIVAL
复制标题
DOI：
10.1094/phyto-84-883
复制
发表时间：
1994-09-01
期刊：
影响因子：
3.2
通讯作者：
LAZAROVITS, G
中科院分区：
文献类型：
--
作者：
HAWKE, MA;LAZAROVITS, G
本文介绍了大丽轮枝菌的实验室培养和单个微菌核的收获、分离和处理方法。大量生产的MS在几个温度下储存可保持12个月或更长时间的高、均匀的活力。因此，单批MS可用于微生物相互作用对MS存活影响的长期实验。通过湿筛从半固体培养基中收获MS，与砂混合干燥而不结块，并通过干筛分选成大小类别。实验后，将单个MS在无菌针头上转移到琼脂平板上标记的25个正方形之一中，并监测萌发和菌落生长。处理的致死效应由死亡MS的数量反映。亚致死效应也可以测量为菌落生长的减少，因为较大的MS（>75 μ m）比较小的MS表现出更快、更同步的发芽，并且产生更大的菌落。
A method for the laboratory culture of Verticillium dahliae and the harvest, isolation, and manipulation of single microsclerotia (MS) is outlined. MS produced in large numbers and stored at several temperatures maintained high, uniform viability for 12 mo or more. Thus, a single lot of MS could be used in long-term experiments on the effect of microbial interaction on MS survival. The MS were harvested from semisolid media by wet sieving, mixed with sand to dry without clumping, and sorted into size classes by dry sieving. After experimentation, single MS were transferred on the tip of a sterile needle into one of 25 squares marked on an agar plate, and germination and colony growth were monitored. The lethal effect of the treatment was reflected by the number of dead MS. Sublethal effects could also be measured as a reduction in colony growth because larger MS (>75 mu m) exhibited faster, more synchronous germination and produced larger colonies than smaller MS.