Cavitation in the mouse preimplantation embryo: Na/K-ATPase and the origin of nascent blastocoele fluid.
Cavitation in the mouse preimplantation embryo: Na/K-ATPase and the origin of nascent blastocoele fluid.
复制标题
小鼠植入前胚胎中的空化：Na/K-ATP酶和新生囊胚液的起源。
DOI：
10.1016/0012-1606(84)90290-2
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发表时间：
1984
影响因子：
2.7
通讯作者：
Wiley,LM
中科院分区：
文献类型：
--
作者：
Wiley,LM
这项研究测试了Na K-ATPase活性可能通过促进某些细胞器定位于相对的细胞边界、产生新生胚胎液和空化而参与小鼠胚泡形成的形态发生方面的命题。推测这种Na K-ATPase活性对不同浓度的外源K(Ko)和哇巴因敏感，前者会改变质膜电位，后者会直接改变Na K-ATPase功能。在不同浓度的Ko和/或哇巴因中培养40小时，观察它们形成囊腔(空洞)的能力和将线粒体定位于相对细胞边界的能力。当Ko从对照的6.0 mm减小到0.6 mm时，空化加速，当Ko增加到25 mm时，空化延迟。Ko为6.0 mm时，10−5M哇巴因加速空化，10−4M哇巴因延迟空化，40小时培养结束时空化胚胎总数减少。当Ko为0.6 mm时，10−5M哇巴因可延迟空化，而10−4M哇巴因则几乎完全抑制空化。当Ko增加到25 mm时，10−5M哇巴因又加速了空化，而10−4M哇巴因则延迟了空化而不是抑制了空化。电子显微镜水平上的形态计量学分析表明，在加速或延迟空化的条件下，线粒体到相对细胞边界的距离发生了变化，这些变化在桑椹胚的内外细胞中是不同的。这些观察结果与Na K-ATPase活性可能参与细胞器向相对细胞边界的定位、新生囊胚液的产生以及小鼠囊胚发育过程中的空化有关的命题一致。
This study tested the proposition that Na K-ATPase activity could be involved in the morphogenetic aspects of mouse blastocyst formation by facilitating the localization of certain organelles to apposed cell borders, the production of nascent blastocoele fluid, and cavitation. It was assumed that such Na K-ATPase activity would be sensitive to varying concentrations of external K (K o)—which would alter plasma membrane potentials—and to ouabain—which would directly alter Na K-ATPase function. Morulae were cultured for 40 hr in varying concentrations of K o and/or ouabain and were observed for their ability to form blastocoeles (cavitate) and to localize mitochondria to apposed cell borders. Cavitation was accelerated when K o was decreased from the control value of 6.0 to 0.6 mM and was delayed when K o was increased to 25 mM. With K o at 6.0 mM, 10− 5 M ouabain accelerated cavitation while 10− 4 M ouabain delayed cavitation and reduced the total number of embryos that cavitated by the end of the 40-hr culture period. With K o at 0.6 mM, 10− 5 M ouabain now delayed cavitation while 10− 4 M ouabain almost completely inhibited it. When K o was increased to 25 mM, 10− 5 M ouabain again accelerated cavitation while 10− 4 M ouabain delayed—rather than inhibited—cavitation. Morphometric analyses at the electron microscopic level showed changes in the distances of mitochondria from apposed cell borders with conditions that accelerated or delayed cavitation and these changes differed for inside and outside cells of the morula. These observations are consistent with the proposition that Na K-ATPase activity could be involved in the localization of organelles to apposed cell borders, the production of nascent blastocoele fluid, and in cavitation during mouse blastocyst development.