Electron microscopic immunocytochemical localization of storage proteins in Vicia faba seeds.
Electron microscopic immunocytochemical localization of storage proteins in Vicia faba seeds.
复制标题
蚕豆种子中储存蛋白的电子显微镜免疫细胞化学定位。
DOI：
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发表时间：
1982
影响因子：
6.6
通讯作者：
E. Weber
中科院分区：
文献类型：
--
作者：
U. zur Nieden;D. Neumann;R. Manteuffel;E. Weber
The main storage proteins legumin and vicilin were located by immuno-labeling with protein A-gold in formaldehyde fixed tissues of Vicia faba seeds at different developmental stages. Both proteins were found to be accumulated together in the same protein bodies. Besides these organelles only the rough endoplasmic reticulum (ER) is labeled in particular developmental stages of the cotyledons. A fraction of proteins accumulated in the vacuoles at earlier stages of development also represents storage proteins. Besides the formation of protein bodies by the ER a second route of origination from vacuoles is proposed for Vicia faba seeds at early developmental stages.
