BCL6 alternative breakpoint region break and homozygous deletion of 17q24 in the nodular lymphocyte predominance type of Hodgkin's lymphoma-derived cell line DEV
BCL6 alternative breakpoint region break and homozygous deletion of 17q24 in the nodular lymphocyte predominance type of Hodgkin's lymphoma-derived cell line DEV
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DOI：
10.1016/j.humpath.2006.01.018
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发表时间：
2006-06-01
期刊：
影响因子：
3.3
通讯作者：
van den Berg, Anke
中科院分区：
文献类型：
--
作者：
Atayar, Cigdem;Kok, Klaas;van den Berg, Anke
DEV是唯一来源于结节性淋巴细胞优势型霍奇金淋巴瘤（NLPHL）的细胞系；然而，缺乏关于这种独特细胞系的遗传和免疫表型的综合报道。我们分析了DEV的免疫表型和遗传畸变。免疫染色显示CD45、CD20、CD22、CD79a、IgA2、CD80、CD86、CD74和BCL6阳性。细胞遗传学上，DEV有复杂的3号染色体易位，包括7、14和22号染色体。对der(3)t(3;14)(p14;q32)t(3;22)（q27;q11.2）的3q27断点的详细分析显示BCL6可选断点区域有一个断点。利用阵列比较基因组杂交技术，在17q24.1-24.2位点发现了一个3兆碱基纯合缺失。荧光原位杂交显示存在2个17号染色体同源物，每个同源物都携带一个小的间质缺失。纯合缺失区域两侧的8个微卫星标记均显示纯合模式，表明亲本等位基因丢失了一个。D17S1809和D17S1816不能用DEV DNA扩增，与纯合缺失片段的位置保持一致。综上所述，DEV的免疫表型与NLPHL病例的肿瘤细胞淋巴细胞和组织细胞一致。我们通过BCL6蛋白的核染色和在可选断点区域的断点的存在证明了BCL6基因的参与，并在17q24处发现了一个纯合缺失区域。(c) 2006爱思唯尔公司版权所有。
DEV is the only cell line derived from nodular lymphocyte predominance type of Hodgkin's lymphoma (NLPHL); however, a comprehensive report about the genetic and immunophenotypic profile of this unique cell line is lacking. We analyzed DEV with respect to immunophenotype and genetic aberrations. The immunostaining revealed positivity for CD45, CD20, CD22, CD79a, IgA2, CD80, CD86, CD74, and BCL6. Cytogenetically, DEV has complex chromosome 3 translocations involving chromosomes 7, 14, and 22. A detailed analysis of the 3q27 breakpoint of the der(3)t(3;14)(p14;q32)t(3;22)(q27;q11.2) revealed a break in the BCL6 alternative breakpoint region. Using array comparative genomic hybridization, a 3-megabase homozygous deletion at 17q24.1-24.2 was identified. Fluorescence in situ hybridization indicated the presence of 2 chromosome 17 homologues, each of which carried a small interstitial deletion. Eight microsatellite markers flanking the homozygously deleted region all showed a homozygous pattern suggesting loss of one of the parental alleles. D17S1809 and D17S1816 could not be amplified using DEV DNA, in keeping with a location within the homozygously deleted segment. In conclusion, DEV has an immunophenotype that is consistent with the neoplastic cells of NLPHL cases, the lymphocytic and histiocytic cells. We demonstrated involvement of the BCL6 gene based on the presence of a breakpoint in the alternative breakpoint region and nuclear staining for BCL6 protein and identified a homozygously deleted region at 17q24. (c) 2006 Elsevier Inc. All rights reserved.