LOCALIZATION OF HISTIDINE DECARBOXYLASE MRNA IN RAT BRAIN
LOCALIZATION OF HISTIDINE DECARBOXYLASE MRNA IN RAT BRAIN
复制标题
DOI：
10.1016/1044-7431(90)90036-4
复制
发表时间：
1990-01-01
影响因子：
3.5
通讯作者：
MILLHORN D E
中科院分区：
文献类型：
--
作者：
BAYLISS D A;WANG Y-M;MILLHORN D E
最近克隆了组胺合成酶--胎鼠肝脏组氨酸脱羧酶(HDC)的基因，为在分子水平上研究中枢组胺能系统奠定了基础。为此，用Northern印迹和原位杂交分析了HDC mRNA在大鼠脑内的区域和细胞分布。Northern blotts检测到3个杂交种的迁移量分别为1.6 kb、2.6 kb和3.5 kb。胎儿肝脏HDC基因的主要(2.6kb)和次要(3.5kb)基因在间脑高水平表达，在海马区仅可检测到，但在其他脑区不表达。相比之下，除了Olfactor灯泡外，所有被检查的大脑区域都存在1.6kb的物种。通过原位杂交，在下丘脑中发现了含有HDC mRNA的细胞；在包括海马区在内的其他区域没有检测到含有HDC mRNA的细胞。表达HDC mRNA的下丘脑神经元定位于结节乳头核的各个方面，这一结果与以前的免疫组织化学结果一致。
The recent cloning of a cDNA encoding fetal rat liver histidine decarboxylase (HDC), the synthesizing enzyme for histamine, allows the study of the central histaminergic system at the molecular level. To this end, Northern blot and in situ hybridization analyses were used to determine the regional and cellular distribution of neurons which express HDC mRNA in rat brain. Three hybridizing species which migrates as 1.6-, 2.6-, and 3.5-kb RNA were identified with Northern blots. The major (2.6 kb) and minor (3.5 kb) species, characteristic of HDC mRNA in fetal liver, were expressed at high levels in diencephalon and at just detectable levels in hippocampus, but not in other brain regions. In contrast, the 1.6-kb species was present in all brain regions examined except the olfactor bulb. Cells which contain HDC mRNA were found by in situ hybridization in the hypothalamus; HDC mRNA-containing cells were not detected in other areas, including the hippocampus. Hypothalamic neurons which express HDC mRNA were localized to all aspects of the tuberomammillary nucleus, a result consistent with previous immunohistochemical findings.