A NOVEL ENDORIBONUCLEASE CLEAVES AT A PRIMING SITE OF MOUSE MITOCHONDRIAL-DNA REPLICATION
A NOVEL ENDORIBONUCLEASE CLEAVES AT A PRIMING SITE OF MOUSE MITOCHONDRIAL-DNA REPLICATION
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DOI：
10.1002/j.1460-2075.1987.tb04770.x
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发表时间：
1987-02-01
期刊：
影响因子：
11.4
通讯作者：
CLAYTON, DA
中科院分区：
文献类型：
--
作者：
CHANG, DD;CLAYTON, DA
在小鼠线粒体重链DNA复制起点的引发受到来自轻链启动子的转录物的影响。从RNA合成到DNA合成的转变发生在转录起始位点下游75至165个核苷酸之间的特定位置。我们已经确定和部分纯化的内切核酸酶活性，准确地切割RNA附近的这些过渡位点之一，这一发现意味着特定的RNA加工在DNA复制中的作用。切割产物具有5”-磷酰基末端和3”-羟基末端。使用小鼠或人线粒体核糖核酸内切酶与含有人或小鼠线粒体重链DNA复制起点的序列的底物的异源性测定表明，切割位点的选择由与实际切割位置相邻的序列指导。
Priming at the mouse mitochondrial origin of heavy-strand DNA replication is effected by transcripts from the lightstrand promoter. The transition from RNA synthesis to DNA synthesis occurs at specific locations between 75 and 165 nucleotides downstream from the transcriptional initiation site. We have identified and partially purified an endonucleolytic activity that cleaves RNA accurately near one of these transition sites; this finding implies a role of specific RNA processing in DNA replication. Cleavage products possess 5''-phosphoryl and 3''-hydroxyl termini. Heterologous assays using mouse or human mitochondrial endoribonuclease with substrates containing the sequences of the human or mouse mitochondrial origins of heavy-strand DNA replication suggest that selection of the cleavage site is guided by sequences adjacent to the actual position of cleavage.