Object location memory in mice: Pharmacological validation and further evidence of hippocampal CA1 participation
Object location memory in mice: Pharmacological validation and further evidence of hippocampal CA1 participation
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DOI：
10.1016/j.bbr.2009.06.005
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发表时间：
2009-12-01
影响因子：
2.7
通讯作者：
Takahashi, Reinaldo Naoto
中科院分区：
文献类型：
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作者：
Assini, Fabricio Luiz;Duzzioni, Marcelo;Takahashi, Reinaldo Naoto
目标定位任务（奥尔特）是一种记忆依赖性的模型。尽管这一应用，既没有一个一致的药理学验证NMDA受体的调制，也没有海马参与小鼠的评价。在奥尔特中，将小鼠放置在具有两个相同物体的开放视野中3 min，并且在延迟30、90、180或360 min后，将一个物体移动到新位置，并记录在新位置（新位置）和旧位置（熟悉位置）探索物体所花费的时间。我们的研究结果表明，小鼠能够辨别物体的位置时，无论是90或180分钟后的训练。腹腔注射NMDA受体拮抗剂MK 801或乙酰胆碱受体拮抗剂东莨菪碱可引起记忆障碍。另一方面，D-环丝氨酸（NMDA激动剂）或他克林（胆碱酯酶抑制剂）能够改善测试小鼠的记忆力。此外，训练前10分钟在海马CA 1区输注利多卡因阻断了物体位置记忆。总之，这项工作表明，奥尔特是容易调制的NMDA受体，胆碱能神经传递，它是第一个表征参与海马CA 1区，在这项任务。(C)2009爱思唯尔有限公司版权所有。
Object location task (OLT) has been used as a model of hippocampal-dependent memory. Despite this application, there is neither a consistent pharmacological validation of NMDA receptor modulation nor an evaluation of hippocampal participation in mice. In the OLT, mice were placed in the open field with two identical objects for 3 min and, after a delay of 30,90,180 or 360 min, one object was moved to a new location and the time spent exploring the objects in new, (novel) and old (familiar) locations was recorded. Our results showed that the mice were able to discriminate object location when tested either 90 or 180 min after training. Intraperitoneal administration of MK801 (NMDA receptors antagonist) or scopolamine (mACh antagonist) induced amnesic effects. On the other hand, D-cycloserine (NMDA agonist) or tacrine (cholinesterase inhibitor) were able to improve memory in the mice tested. In addition, lidocaine infusion in the hippocampal CA1 region 10 min before training blocked object location memory. In short, this work indicates that OLT is susceptible to modulation of NMDA receptors, cholinergic neurotransmission and it is the first to characterize the participation of the hippocampal CA1 region, in this task. (C) 2009 Elsevier B.V. All rights reserved.