Correlative study of angiogenesis and dynamic contrast-enhanced magnetic resonance imaging features of hepatocellular carcinoma
Correlative study of angiogenesis and dynamic contrast-enhanced magnetic resonance imaging features of hepatocellular carcinoma
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DOI：
10.1080/02841850510021247
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发表时间：
2005-07-01
期刊：
影响因子：
1.3
通讯作者：
Yan, X
中科院分区：
文献类型：
--
作者：
Wang, B;Gao, ZQ;Yan, X
目的：通过分析肝细胞癌（HCC）的微血管密度（MVD）、血管内皮生长因子（VEGF）和P53蛋白表达，探讨动态增强磁共振成像（DCE-MRI）增强模式与血管生成的关系。1998年10月至2000年3月，采用GE Signa 1.5T磁共振扫描机，对30例患者（38个病灶）进行了SE和FMPSPGR序列的MRI检查。所有患者均经组织病理学证实为HCC。回顾性审查/分析了MR图像。30例患者年龄在35 - 65岁之间（平均年龄58岁; 18例男性和12例女性）。行SE序列T1 WI、PDWI、T2 WI扫描。在相同位置获得FMPSPGR序列。在静脉注射Gd-DTPA后，在动脉、门静脉和延迟相进行DCE-MRI。对标本进行CD 34、VEGF和P53的免疫组化染色。抗CD 34抗体染色显示MVD。在蛋白水平上研究HCC病灶的强化特征与肿瘤MVD、VEGF和P53表达的相关性。在动脉期，结果显示：高增强组的MVD（229.76 ± 80.96）高于等强化组（173.09 ± 61.38）和低强化组（153.00 ± 108.58）（P
Purpose: To explore the correlation between contrast-enhancement patterns on dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and angiogenesis by analyzing microvessel density (MVD), vascular endothelial growth factor ( VEGF), and P53 protein expression in hepatocellular carcinoma (HCC).Material and Methods: MRI was performed with a GE Signa 1.5T MR scanner using SE and FMPSPGR sequences in 30 patients ( 38 lesions) during the period October 1998 to March 2000. All had histopathologically proven HCC. MR images were reviewed/ analyzed retrospectively. The 30 patients were between 35 and 65 years of age ( average age 58; 18 M and 12 F). SE T1WI, PDWI, and T2WI were acquired initially. The FMPSPGR sequence was acquired in the same position. The DCE-MRI was performed in the arterial, portal vein, and delay phase after a bolus injection of Gd-DTPA. The specimens were stained immunohistochemically for CD34, VEGF, and P53. MVD was highlighted by anti-CD34 antibody staining. The enhancement features of HCC lesions were studied correlatively with the tumor MVD, VEGF, and P53 expression at protein level.Results: In the arterial phase, the results showed that MVD of HCC in the high-enhancement group (229.76 +/- 80.96) was higher than that in the equal-enhancement (173.09 +/- 61.38) and low- enhancement groups (153.00 +/- 108.58) ( P