Large‐scale investigation of zoonotic viruses in the era of high‐throughput sequencing
Large‐scale investigation of zoonotic viruses in the era of high‐throughput sequencing
复制标题
高通量测序时代的人畜共患病毒大规模调查
DOI：
10.1111/1348-0421.13033
复制
发表时间：
2022-10
影响因子：
2.6
通讯作者：
Junna Kawasaki;K. Tomonaga;Masayuki Horie
中科院分区：
文献类型：
--
作者：
Junna Kawasaki;K. Tomonaga;Masayuki Horie
人畜共患疾病对公共卫生和社会经济产生重大影响。据报道，RNA病毒引起了1990年至2010年记录的约94%的人畜共患病，强调了研究动物中RNA病毒的重要性。此外，据估计，数十万种能够感染人类的动物病毒尚未被发现，这警告我们对病毒多样性的理解不足。高通量测序（HTS）使得能够以相对较小的偏倚鉴定病毒感染。HTS使用有症状和无症状的动物样本进行病毒搜索，发现了隐藏的病毒感染。这篇综述介绍了使用HTS的病毒搜索的历史，目前的分析局限性和未来的潜力。我们主要总结了最近关于病毒感染的大规模调查的研究，这些调查使用了来自公共数据库的HTS数据。此外，考虑到未培养病毒的积累，我们讨论了使用各种方法将病毒序列与其表型联系起来的当前研究和挑战：进行数据分析，开发预测建模或实施高通量病毒学实验平台。我们相信，这篇文章为使用HTS技术对潜在的人畜共患病病毒进行大规模调查提供了未来的方向。
Zoonotic diseases considerably impact public health and socioeconomics. RNA viruses reportedly caused approximately 94% of zoonotic diseases documented from 1990 to 2010, emphasizing the importance of investigating RNA viruses in animals. Furthermore, it has been estimated that hundreds of thousands of animal viruses capable of infecting humans are yet to be discovered, warning against the inadequacy of our understanding of viral diversity. High‐throughput sequencing (HTS) has enabled the identification of viral infections with relatively little bias. Viral searches using both symptomatic and asymptomatic animal samples by HTS have revealed hidden viral infections. This review introduces the history of viral searches using HTS, current analytical limitations, and future potentials. We primarily summarize recent research on large‐scale investigations on viral infections reusing HTS data from public databases. Furthermore, considering the accumulation of uncultivated viruses, we discuss current studies and challenges for connecting viral sequences to their phenotypes using various approaches: performing data analysis, developing predictive modeling, or implementing high‐throughput platforms of virological experiments. We believe that this article provides a future direction in large‐scale investigations of potential zoonotic viruses using the HTS technology.