The effect of cis-diamminedichloroplatinum (II) on cultured human lymphoma cells and its therapeutic implications.
The effect of cis-diamminedichloroplatinum (II) on cultured human lymphoma cells and its therapeutic implications.
复制标题
顺式二氯二氨铂 (II) 对培养的人淋巴瘤细胞的影响及其治疗意义。
作者：
B. Drewinko;B. Brown;J. Gottlieb
通过集落形成技术估计，用增加浓度的顺式二胺二氯铂（DDP）治疗异步人淋巴瘤细胞1小时后，其存活率明显下降。当处理时间延长8小时时，观察到5µg/ml浓度的杀伤效果（超过3 log几十）与50µg/ml与细胞孵育1小时所获得的效果相似。这一发现提示，长期低剂量输注DDP可获得较好的抗肿瘤效果和较低的毒性作用。
Asynchronous human lymphoma cells treated for 1 hr with increasing concentrations of cis -diamminedichloroplatinum(II) (DDP) revealed a marked decrease in survival as estimated by the colony-forming technique. When the treatment was extended for 8 hr, a killing effect (more than 3 log decades) was observed for a concentration of 5 µg/ml similar to that obtained with 50 µg/ml incubated with the cells for 1 hr. This finding suggests that better antitumor results with less toxic effects may be obtained clinically by prolonged infusion of low doses of DDP.
Synchronized lymphoma cells showed no significant degree of cell cycle stage sensitivity to DDP. The drug kills cells with similar efficiency in all stages of the cell cycle. No killing effect was elicited after incubation of the cells with spironolactone, a compound said to protect the kidneys from the toxic effect of heavy metals. However, simultaneous incubation of spironolactone and DDP did not prevent the lethal action of the second drug. If spironolactone is proven an inhibitor of DDP nephrotoxicity, it will become a valuable addition to the treatment of human neoplasias with DDP.