High-resolution reconstruction of the beating zebrafish heart
High-resolution reconstruction of the beating zebrafish heart
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DOI：
10.1038/nmeth.3037
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发表时间：
2014-09-01
期刊：
影响因子：
48
通讯作者：
Huisken, Jan
中科院分区：
文献类型：
--
作者：
Mickoleit, Michaela;Schmid, Benjamin;Huisken, Jan
心脏的连续运动使得很难在体内捕获该器官的高分辨率图像。我们开发了基于高速选择性平面照明显微镜（SPIM）的工具，为跳动的斑马鱼心脏提供原始视图。我们捕获三维心脏动力学与采集后同步的多视图电影堆栈，获得静态高分辨率重建短暂停止心脏与光遗传学和解决非周期性现象的高速体积扫描与液体透镜。
The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.