Interactive effect of brassinosteroids and cytokinins on growth, chlorophyll, monosaccharide and protein content in the green alga Chlorella vulgaris (Trebouxiophyceae)
Interactive effect of brassinosteroids and cytokinins on growth, chlorophyll, monosaccharide and protein content in the green alga Chlorella vulgaris (Trebouxiophyceae)
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DOI：
10.1016/j.plaphy.2014.04.009
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发表时间：
2014-07-01
影响因子：
6.5
通讯作者：
Piotrowska-Niczyporuk, Alicja
中科院分区：
文献类型：
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作者：
Bajguz, Andrzej;Piotrowska-Niczyporuk, Alicja
油菜素内酯（BRs）（油菜素内酯，BL； 24-表油菜素内酯，24-epiBL； 28-同型油菜素内酯，28-homoBL；卡斯特酮，CS； 24-表油菜素，24-epiCS； 28-同型卡斯特酮，28-homoCS）与腺嘌呤(反式玉米素，tZ；研究了动素（Kin）和苯脲型（1,3-二苯脲，DPU）细胞分裂素（CKs）对小球藻（Chlorella vulgaris）细胞数量、植物激素水平以及叶绿素、单糖和蛋白质含量的调控作用。小球藻对ck的敏感性依次为：10 nM tZ > 100 nM Kin >1 μ M DPU。外源应用的BRs在浓度为10 nM时在藻类细胞中具有最高的生物活性。其中，BL活性最高，28-homoCS -活性最低。在外源10 nM tZ处理的C vulgaris培养中，所有内源BRs的水平均显著提高了27-46%。可以推测，ck可能是通过诱导内源性BR的积累来刺激C vulgaris的BR活性。ck与BRs协同作用，增加了C的细胞数量和内源蛋白质、叶绿素和单糖的积累。结果表明，BL与tZ联合处理对藻类生长和生化指标的刺激作用最大，而28-homoCS和DPU处理对藻类生长和生化指标的刺激作用最小。然而，无论使用哪种BRs和ck的混合物，单细胞绿藻的细胞数量和代谢物积累的显著增加都高于任何单一植物激素处理的培养水平。(C) 2014 Elsevier Masson SAS。版权所有。
Interaction between brassinosteroids (BRs) (brassinolide, BL; 24-epibrassinolide, 24-epiBL; 28-homobrassinolide, 28-homoBL; castasterone, CS; 24-epicastasterone, 24-epiCS; 28-homocastasterone, 28-homoCS) and adenine- (trans-zeatin, tZ; kinetin, Kin) as well as phenylurea-type (1,3-diphenylurea, DPU) cytokinins (CKs) in the regulation of cell number, phytohormone level and the content of chlorophyll, monosaccharide and protein in unicellular green alga Chlorella vulgaris (Trebouxiophyceae) were examined. Chlorella vulgaris exhibited sensitivity to CKs in the following order of their stimulating properties: 10 nM tZ > 100 nM Kin >1 mu M DPU. Exogenously applied BRs possessed the highest biological activity in algal cells at concentration of 10 nM. Among the BRs, BL was characterized by the highest activity, while 28-homoCS - by the lowest. The considerable increase in the level of all endogenous BRs by 27-46% was observed in C vulgaris culture treated with exogenous 10 nM tZ. It can be speculated that CKs may stimulate BR activity in C vulgaris by inducing the accumulation of endogenous BRs. CKs interacted synergistically with BRs increasing the number of cells and endogenous accumulation of proteins, chlorophylls and monosaccharides in C vulgaris. The highest stimulation of algal growth and the contents of analyzed biochemical parameters were observed for BL applied in combination with tZ, whereas the lowest in the culture treated with both 28-homoCS and DPU. However, regardless of the applied mixture of BRs with CKs, the considerable increase in cell number and the metabolite accumulation was found above the level obtained in cultures treated with any single phytohormone in unicellular green alga C vulgaris. (C) 2014 Elsevier Masson SAS. All rights reserved.