Robust ΦC31-Mediated Genome Engineering in Drosophila melanogaster Using Minimal attP/attB Phage Sites.
Robust ΦC31-Mediated Genome Engineering in Drosophila melanogaster Using Minimal attP/attB Phage Sites.
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DOI：
10.1534/g3.118.200051
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发表时间：
2018-05-04
期刊：
影响因子：
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通讯作者：
Mann RS
中科院分区：
文献类型：
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作者：
Voutev R;Mann RS
有效的基因组工程应导致所需的基因座变化，对基因组本身的不利影响最小。然而，具有定点重组酶识别位点的侧翼基因座，例如噬菌体ΦC31整合酶的那些，允许一旦靶向特定基因座，就以迭代方式创建用于盒交换和基因组区域操作的平台。在这里，我们表明，基因组位点工程与反向最小噬菌体ΦC31 attP/attB位点可以进行有效的重组酶介导的盒交换（RMCE）的果蝇黑腹果蝇。
Effective genome engineering should lead to a desired locus change with minimal adverse impact to the genome itself. However, flanking loci with site-directed recombinase recognition sites, such as those of the phage ΦC31 integrase, allows for creation of platforms for cassette exchange and manipulation of genomic regions in an iterative manner, once specific loci have been targeted. Here we show that a genomic locus engineered with inverted minimal phage ΦC31 attP/attB sites can undergo efficient recombinase-mediated cassette exchange (RMCE) in the fruit fly Drosophila melanogaster.