Geometric parameters of pipettes and membrane patches
Geometric parameters of pipettes and membrane patches
复制标题
移液器和膜片的几何参数
DOI：
10.1007/978-1-4615-7858-1_2
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
E. Neher
中科院分区：
文献类型：
--
作者：
B. Sakmann;E. Neher
贴片电流测量的对象是一个小的膜贴片和一个尺寸相当的移液管尖端。两者都是光学显微镜分辨率的极限。在实验过程中，只有很少的情况下才有可能“看到”膜斑，即使这样，也不可能或很难定量评估其大小。本章的目的是提供一些提示，通过光学和电子显微镜以及电学测量的组合来估计贴片移液器的尖端几何形状和贴片尺寸。然而，应该强调的是，即使实验者控制的所有参数保持恒定，尖端几何形状和贴片尺寸也有很大的变化。因此，所提供的数字只应视为数量级估计数。
The object of the patch current measurement is a small membrane patch and a pipette tip of comparable dimensions. Both are at the limit of resolution of the optical microscope. Only rarely is it possible to “see” the membrane patch during the experiment, and even then quantitative evaluation of its size is impossible or difficult. The purpose of this chapter is to provide some hints toward estimation of the tip geometry of patch pipettes and of patch sizes by a combination of light and electron microscopy and electrical measurements. It should be emphasized, however, that there are wide variations in tip geometry and patch sizes, even if all the parameters that are under control of the experimenter are kept constant. The numbers given should therefore only be considered as order-of-magnitude estimates.