USE OF ISOZYME-MARKED SEGMENTS FROM WILD BARLEY (HORDEUM-SPONTANEUM) IN BARLEY BREEDING
USE OF ISOZYME-MARKED SEGMENTS FROM WILD BARLEY (HORDEUM-SPONTANEUM) IN BARLEY BREEDING
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DOI：
10.1111/j.1439-0523.1988.tb00254.x
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发表时间：
1988-06-01
期刊：
影响因子：
2
通讯作者：
ORAM, RN
中科院分区：
文献类型：
--
作者：
BROWN, AHD;MUNDAY, J;ORAM, RN
本文对56份来自以色列不同生境的天然Hordeum spontanum进行了杂交，并将其后代回交3次与H. vulgare cv杂交。从“Clipper”24个同工酶位点中选择1个标记的染色体片段，转化成84个与“Clipper”接近等基因的回交系。每条细胞系的同工酶标记片段为纯合子。这些品系在初步和后期的田间试验中进行了评价。这些品系的产量、麦芽提取物百分比和籽粒大小接近或等于，有时甚至大大超过“克利伯”和最近的商业品种。将三个同工酶标记的片段成对组合，并将所得到的三个双标记系与重组亲本、单标记系以及来自同一f2阵列的“Clipper”重组系进行比较。单片段的加性效应对产量和籽粒大小的影响不显著，而6个相互作用中有一半是显著的，但在不利的方向上。结果表明，近等基因系可以鉴定出对提高产量有用的野生大麦种质片段，但来自不同生境的近等基因系对可能存在互作不利的情况。
Fifty-six accessions of Hordeum spontaneum from various Israeli habitats were hybridized with, and their progeny backcrossed three times to H. vulgare cv. ''Clipper'', Selected chromosome segments marked by one of 24 isozyme loci were transferred to form 84 backcross lines which were nearly isogenic with ''Clipper''. Each line was made homozygous for a single isozyme-marked segment. These lines were evaluated in preliminary and advanced field trials. The yield, malt extract percentage and grain size of the lines approached or equalled, and sometimes significantly surpassed, that of ''Clipper'' and more recent commerical cultivars. Three isozyme-marked segments were combined in pairs and the three resultant doubly-marked lines were compared with the reconstituted parental, singly-marked lines and with the ''Clipper'' recombinant from the same F2-array. The additive effects of single segments on yield or grain size were not significant, whereas half of the six interactions were significant, but in the unfavourable direction. It was concluded that near-isogenic lines can identify segments of wild barley germplasm that are useful for improving yield, but that pairs of these from different habitats may interact unfavourably.