Fetal and neonatal exposure to the mycotoxin zearalenone induces phenotypic alterations in adult rat mammary gland
Fetal and neonatal exposure to the mycotoxin zearalenone induces phenotypic alterations in adult rat mammary gland
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DOI：
10.1016/j.fct.2010.07.012
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发表时间：
2010-10-01
影响因子：
4.3
通讯作者：
Le Jan, Christian
中科院分区：
文献类型：
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作者：
Belli, Patrick;Bellaton, Claire;Le Jan, Christian
玉米赤霉烯酮是一种广泛存在于谷类食品中的霉菌毒素。我们质疑，在已知的关键暴露期（如胎儿期和生命最初几天），以与人类平均每日摄入量相匹配的剂量给药这种霉菌毒素，是否会对啮齿动物的乳腺发育产生影响。Wistar雌性大鼠在胎儿生命的最后14天和出生后的前5天暴露于玉米赤霉烯酮（0.2 μ g/kg ~ 5 mg/kg）。通过形态学分析和免疫化学检查乳腺组织的发育和成熟情况。在PND 30时，所有暴露玉米赤霉烯酮的雌花的顶芽平均长度均显著增加（p < 0.05）。通过组织切片评分，1 mg/kg剂量组的乳腺组织分化程度明显高于对照组（p < 0.05）。在PND 180时，所有玉米赤霉烯酮治疗组的乳腺组织分化程度均较高（p < 0.05）。6个月时，暴露于5 mg/kg玉米赤霉烯酮的18只雌性中有4只出现乳腺增生病变。在子宫内和新生儿期以低至0.2 μ g/kg的剂量给药玉米赤霉烯酮诱导表型改变，表明玉米赤霉烯酮可能有助于诱导乳腺内分泌紊乱。(C) 2010 Elsevier Ltd.版权所有。
Zearalenone is a mycotoxin that is widespread in cereal food. We questioned whether this mycotoxin, administered during known critical exposure periods such as the fetal period and the first days of life, at doses compatible with mean daily intake in humans, could have an effect on mammary gland development in rodents. Wistar female rats were exposed to zearalenone (0.2 mu g/kg to 5 mg/kg) during the last 14 days of fetal life and the first 5 post-natal days (PND). The mammary tissue was examined for development and maturation by morphologic analyses and immunochemistry. At PND 30, the mean length of terminal buds was significantly enhanced in all of the zearalenone-exposed females (p < 0.05). The mammary tissue, as evaluated by scoring of tissue slides, was significantly more differentiated in the 1 mg/kg treated group than in controls (p < 0.05). At PND 180, mammary tissue was more differentiated in all of the zearalenone treated groups (p < 0.05). At six months, 4 of 18 females exposed to 5 mg/kg of zearalenone presented mammary hyperplasia lesions. The induction of phenotypic alterations by zearalenone administered in utero and in the neonatal period at doses as low as 0.2 mu g/kg suggests that zearalenone could contribute to the induction of breast endocrine disorders. (C) 2010 Elsevier Ltd. All rights reserved.