Spatial relationship between bone formation and mechanical stimulus within cortical bone: Combining 3D fluorochrome mapping and poroelastic finite element modelling.
Spatial relationship between bone formation and mechanical stimulus within cortical bone: Combining 3D fluorochrome mapping and poroelastic finite element modelling.
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DOI：
10.1016/j.bonr.2018.02.003
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发表时间：
2018-06
期刊：
影响因子：
2.5
通讯作者：
Shefelbine SJ
中科院分区：
文献类型：
--
作者：
Carrieroa A;Pereirab AF;Wilson AJ;Castagno S;Javaheri B;Pitsillides AA;Marenzana M;Shefelbine SJ
骨是一种动态的组织，并适应其结构，以响应生物和机械因素。在这里，我们调查皮质骨形成的空间控制的局部力学环境中的小鼠胫骨轴向负荷模型（C57 BL/6）。我们通过对整个骨骼进行“切片和视图”3D荧光染料映射来获得新骨形成的3D位置，并将这些位置与使用有限元模型估计的高流体速度或应变能密度的区域进行比较，使用数字图像相关性使用离体获取的离体骨表面应变图进行验证。为了进行比较，在整个皮质表面上创建胫骨骨内膜和骨膜表面上的平均骨形成和峰值机械刺激的2D图。结果表明，骨形成的骨膜和骨内膜表面的高流体流量的区域。峰值应变能密度仅预测骨膜骨的形成。了解机械刺激如何在空间上与响应于负荷的皮质骨形成区域相关，最终将指导负荷方案治疗以维持或恢复骨骼病理学中特定部位的骨量。加载后新骨形成的3D空间表示通过整个小鼠胫骨的荧光染料映射显示。新骨形成的区域在空间上与FE模型中的高应变和流体机械刺激的区域相关联。FE模型用使用数字图像相关性离体确定的骨表面上的应变进行验证。新骨形成的区域共同定位在峰值流体流动的位点，峰值应变能密度仅能预测骨膜骨形成
Bone is a dynamic tissue and adapts its architecture in response to biological and mechanical factors. Here we investigate how cortical bone formation is spatially controlled by the local mechanical environment in the murine tibia axial loading model (C57BL/6). We obtained 3D locations of new bone formation by performing ‘slice and view’ 3D fluorochrome mapping of the entire bone and compared these sites with the regions of high fluid velocity or strain energy density estimated using a finite element model, validated with ex-vivo bone surface strain map acquired ex-vivo using digital image correlation. For the comparison, 2D maps of the average bone formation and peak mechanical stimulus on the tibial endosteal and periosteal surface across the entire cortical surface were created. Results showed that bone formed on the periosteal and endosteal surface in regions of high fluid flow. Peak strain energy density predicted only the formation of bone periosteally. Understanding how the mechanical stimuli spatially relates with regions of cortical bone formation in response to loading will eventually guide loading regime therapies to maintain or restore bone mass in specific sites in skeletal pathologies. 3D spatial representation of new bone formation after loading is shown by fluorochrome mapping of the entire mouse tibia Regions of new bone formation spatially associate with regions of high strain and fluid mechanical stimulus in a FE model The FE model was validated with the strains on the bone surface determined ex-vivo using digital image correlation Regions of new bone formation co-localize in sites of peak fluid flow, both endosteally and periosteally Peak strain energy density was able to predict only periosteal bone formation