Xbves is a regulator of epithelial movement during early Xenopus laevis development
Xbves is a regulator of epithelial movement during early Xenopus laevis development
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DOI：
10.1073/pnas.0506095103
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发表时间：
2006-01-17
影响因子：
11.1
通讯作者：
Bader, D
中科院分区：
文献类型：
--
作者：
Ripley, AN;Osler, ME;Bader, D
Elves/Popla是一种在胚胎上皮和横纹肌中表达的独特的、高度保守的膜整合蛋白。虽然研究已经提出了上皮形态发生的作用，Bves/popla在发育中的功能是完全未知的。在这里，我们表明，非洲爪蟾Bves（Xbves）的RNA和蛋白质表达的上皮细胞的早期胚胎。将Xbves转染到非粘附小鼠L细胞中赋予细胞/细胞粘附。通过向两细胞胚胎中注射吗啉代来全面抑制Xbves功能，通过解除对上皮细胞外生和退化运动的调节来阻止原肠胚形成时的发育。在A1卵裂球及其衍生物内Xbves活性的克隆抑制完全随机化了其后代在其他正常分化的胚胎内的运动。这些数据表明，精灵/白杨蛋白在上皮形态发生中起着关键作用，特别是在X laevis发育过程中发生的上皮重排所必需的细胞运动中。
Elves/popla is a unique, highly conserved integral membrane protein expressed in embryonic epithelia and striated muscle. Although studies have proposed a role in epithelial morphogenesis, the function of Bves/popla in development is completely unknown. Here we show that Xenopus laevis Bves (Xbves) RNA and protein are expressed in epithelia of the early embryo. Transfection of Xbves into nonadherent mouse L cells confers cell/cell adhesion. Global inhibition of Xbves function by morpholino injection into two-cell embryos arrests development at gastrulation by deregulating the epithelial movements of epiboly and involution. Clonal inhibition of Xbves activity within the A1 blastomere and its derivatives completely randomizes movement of its progeny within otherwise normally differentiating embryos. These data demonstrate that Elves/popla proteins play a critical role in epithelial morphogenesis and, specifically, in the cell movements essential for epithelial rearrangements that occur during X laevis development.