A sensitive, colorimetric immunosensor based on Cu-MOFs and HRP for detection of dibutyl phthalate in environmental and food samples
A sensitive, colorimetric immunosensor based on Cu-MOFs and HRP for detection of dibutyl phthalate in environmental and food samples
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基于 Cu-MOF 和 HRP 的灵敏比色免疫传感器，用于检测环境和食品样品中的邻苯二甲酸二丁酯
DOI：
10.1016/j.talanta.2018.04.023
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发表时间：
2018
期刊：
影响因子：
6.1
通讯作者：
Zhang zhen
中科院分区：
文献类型：
--
作者：
Zhu Nuanfei;Zou Yanmin;Huang Menglu;Dong Shuaibing;Wu Xiangyang;Liang guoxi;Han Zhixiang;Zhang zhen
设计了一种灵敏、灵巧的辣根过氧化物酶(HRP)比色免疫传感器，将金属有机骨架(CuMOFS@AB2)标记在第二抗体(CuMOFS@AB2)上，用于邻苯二甲酸二丁酯(DBP)的检测。在该体系中，当铜-MOFS@Ab2被抗原-抗体(AB1)络合物捕获时，在硝酸(HNO_3)存在下，铜-MOFS会释放出大量的铜(II)，而加入抗坏血酸钠(SA)后，铜(II)会进一步还原为铜(I)，从而抑制HRP催化无色的3，3‘，5，5’-四甲基联苯胺(TMB)生成蓝色氧化TMB(OX TMB)。在优化的条件下，该方法的检出限为1 μg L−1，比常规检测同一抗体的方法低近60倍。加标回收率为87.73-103.4%，变异系数为1.46-5.95%，精密度和重现性良好。该免疫传感器在环境和食品样品中痕量DBP的检测方面具有很大的潜力。
A sensitive and artful colorimetric immunosensor based on horseradish peroxidase (HRP) was designed by labelling metal–organic frameworks (Cu-MOFs) on the second antibody (Cu-MOFs@Ab2) as signal amplification for the detection of trace dibutyl phthalate (DBP). In this system, when Cu-MOFs@Ab2was captured by antigen- primary antibody (Ab1) complex, tremendous Cu(II) will be released from Cu-MOFs in the presence of nitric acid (HNO3), and Cu(II) will be further reduced to Cu(I) after the addition of sodium ascorbate (SA), consequently, inhibiting the HRP to catalyse the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidized TMB (ox TMB). Under the optimized conditions, the limit of detection (LOD) was 1 μg L−1, which was almost 60 times lower than that using a conventional ELISA with the same antibody. In addition, our method showed good accuracy and reproducibility (recoveries of 87.73–103.4%; CV values of 1.46–5.95%) through a spike-recovery analysis. The proposed immunosensor indicated great potential for trace DBP determination from environmental and food samples.