Id1 promotes tumor cell migration in nonsmall cell lung cancers.
Id1 promotes tumor cell migration in nonsmall cell lung cancers.
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DOI：
10.1155/2010/856105
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发表时间：
2010
影响因子：
--
通讯作者：
Alani RM
中科院分区：
文献类型：
--
作者：
Bhattacharya R;Kowalski J;Larson AR;Brock M;Alani RM
Id 1属于Id家族的螺旋-环-螺旋转录因子，与肿瘤进展和转移最相关;然而，其在肺癌中的意义尚未得到广泛探讨。在这里，我们试图评估的表达Id 1在非小细胞肺癌（NSCLC）的试点研究，并确定其在这些肿瘤的诊断和功能的意义。使用Western印迹和定量RT-PCR评估成对的正常和恶性肺组织以及一组NSCLC原发性肿瘤和细胞系的Id 1表达。进行功能测定以评估Id 1在肿瘤细胞生长、迁移和进展中的作用。我们发现，与肺腺癌相比，Id 1在鳞状细胞癌中的表达上调，并且与正常对照相比，Id 1在NSCLC中的表达是可变的。我们还注意到，Id 1在NSCLC细胞中的表达在很大程度上是生长因子依赖性的，并且Id 1在NSCLC细胞中的组成性表达显著增加肿瘤细胞迁移而不影响细胞增殖。我们的结论是，作为肿瘤细胞迁移的介质，Id 1可能是非小细胞肺癌侵袭潜力的指标。
Id1, which belongs to the Id family of helix-loop-helix transcription factors has been most associated with tumor progression and metastatsis; however, its significance in lung cancers has not been extensively explored. Here we seek to evaluate the expression of Id1 in a pilot study of nonsmall-cell lung cancers (NSCLCs) and determine its diagnostic and functional significance in these tumors. Paired normal and malignant lung tissues as well as a panel of NSCLC primary tumors and cell lines were evaluated for Id1 expression using Western blotting and quantitative RT-PCR. Functional assays were performed to evaluate the role of Id1 in tumor cell growth, migration and progression. We find Id1 expression is upregulated in squamous cell carcinoma when compared to adenocarcinoma of the lung and that expression of Id1 versus the normal control is variable in NSCLCs. We also note that Id1 expression in NSCLC cells is largely growth factor dependant and constitutive expression of Id1 in NSCLC cells significantly increases tumor cell migration without affecting cell proliferation. We conclude that Id1, as a mediator of tumor cell migration, may be an indicator of aggressive potential in nonsmall-cell lung cancers.