Modeled channel distributions explain extracellular recordings from cultured neurons sealed to microelectrodes
Modeled channel distributions explain extracellular recordings from cultured neurons sealed to microelectrodes
复制标题
DOI：
10.1109/tbme.2002.805555
复制
发表时间：
2002-12-01
影响因子：
4.6
通讯作者：
Marani, E
中科院分区：
文献类型：
--
作者：
Buitenweg, JR;Rutten, WLC;Marani, E
在嵌入微电极的基质上培养单个神经元的细胞外记录的振幅和形状不仅取决于神经元-电极界面的体积传导特性，还可能取决于神经元膜上电压敏感通道的分布。本文采用有限元模型来量化这些通道分布对神经元-电极接触的影响。神经元密封膜中电压敏感通道的轻微积累或消耗会导致模拟细胞外记录的各种形状和振幅。然而，从细胞外记录中估计通道特异性积累因子可能会受到共同发生的离子流和缺陷密封的阻碍。从培养的神经元-电极界面的实验数据表明，钠通道的消耗和钾通道的积累。
Amplitudes and shapes of extracellular recordings from single neurons cultured on a substrate embedded microelectrode depend not only on the volume conducting properties of the neuron-electrode interface, but might also depend on the distribution of voltage-sensitive channels over the neuronal membrane. In this paper, finite-element modeling is used to quantify the effect of these channel distributions on the neuron-electrode contact. Slight accumulation or depletion of voltage-sensitive channels in the sealing membrane of the neuron results in various shapes and amplitudes of simulated extracellular recordings. However, estimation of channel-specific accumulation factors from extracellular recordings can be obstructed by co-occuring ion currents and defect sealing. Experimental data from cultured neuron-electrode interfaces suggest depletion of sodium channels and accumulation of potassium channels.