Beta-endorphin and neurotensin stimulate in vitro clonal growth of human SCLC cells.
Beta-endorphin and neurotensin stimulate in vitro clonal growth of human SCLC cells.
复制标题
β-内啡肽和神经降压素刺激人 SCLC 细胞的体外克隆生长。
DOI：
10.1016/0014-2999(89)90862-5
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发表时间：
1989
影响因子：
5
通讯作者：
Liu,RH
中科院分区：
文献类型：
--
作者：
Davis,TP;Burgess,HS;Crowell,S;Moody,TW;Culling-Berglund,A;Liu,RH
众所周知，多种神经肽包括神经降压素、表皮生长因子、促肾上腺皮质激素、降钙素、神经激肽A、生长抑素、蛙皮素/胃泌素释放肽（GRP）和f-内啡肽是由神经细胞产生的。肺小细胞癌（SCLC）和源自这些肿瘤的细胞系（Roth和Barchas，1986; Bepler等人，1988年）。仅在一些SCLC细胞系中表征了这些肽中的少数肽的受体（Moody等人，1985; Roth和Barchas，1986; Bepler等人，1988），从而提示特异性肽可以以类似于铃蟾肽/GRP的自分泌方式调节SCLC的生长（Cuttitta等人，1985年）。然而，这些肽中很少有显示刺激SCLC的生长和增殖。由于自分泌假说提出细胞产生并分泌“类胡萝卜素”生长因子，其可以与细胞表面上的膜受体相互作用，进而刺激其自身的生长和增殖，因此并非所有发现存在于SCLC中的肽都作为自分泌肽起作用。使用人类肿瘤克隆形成测定的修改（Hamburger和Salmon，1977），该通信代表了氟内啡肽和神经降压素刺激的第一个证明。
It is well-known that several neuropeptides including neurotensin, epidermal growth factor, adrenocorticotrophic hormone, calcitonin, neurokinin A, somatostatin, bombesin/gastrin-releasing peptide (GRP) and fl-endorphin are produced by! small cell carcinoma of the lung (SCLC) and cell lines derived from these tumors (Roth and Barchas, 1986; Bepler et al., 1988). Receptors for only a few of these peptides have been characterized in some SCLC cell lines (Moody et al., 1985; Roth and Barchas, 1986; Bepler et al., 1988) leading to the suggestion that specific peptides could regulate the growth of SCLC in an autocrine fashion similar to bombesin/GRP (Cuttitta et al., 1985). However, very few of these peptides have been shown to stimulate growth and proliferation of SCLC. Since the autocrine hypothesis proposes that cells produce and secrete a'hormone-like'growth factor which can interact with membrane receptors on the cell surface and in turn act to stimulate its own growth and proliferation, not all peptides found to be present in SCLC function as autocrine peptides. Using a modification of the human tumor clonogenic assay (Hamburger and Salmon, 1977) this communication represents the first demonstration that fl-endorphin and neurotensin stimu-