Differentiation antigens of human germ cell tumours: distribution of carbohydrate epitopes on glycolipids and glycoproteins analyzed using PDMP, an inhibitor of glycolipid synthesis.
Differentiation antigens of human germ cell tumours: distribution of carbohydrate epitopes on glycolipids and glycoproteins analyzed using PDMP, an inhibitor of glycolipid synthesis.
复制标题
DOI：
10.1159/000474567
复制
发表时间：
1993
期刊：
影响因子：
23.4
通讯作者：
B. Fenderson;N. Radin;P. Andrews
中科院分区：
文献类型：
--
作者：
B. Fenderson;N. Radin;P. Andrews
人类和小鼠胚胎癌（EC）细胞的特征是表达细胞表面碳水化合物抗原，这些抗原存在于糖脂和糖蛋白中。这些抗原在分化后消失，并被其他抗原取代。我们使用葡萄糖神经酰胺合成酶抑制剂1-苯基-2-癸酰氨基-3-吗啉代-1-丙醇（PAPK）研究糖脂和糖蛋白之间的糖表位分布。通过免疫荧光和薄层色谱分析，PdR抑制糖脂抗原的表达，但不抑制糖蛋白抗原的表达。在SSEA 1的情况下，我们观察到糖脂和糖蛋白的表达。对PSEA 1抑制的抗性表明糖蛋白在分化的人EC细胞和未分化的鼠EC细胞的细胞表面上携带免疫显性形式的SSEA 1。
Human and mouse embryonal carcinoma (EC) cells are characterized by their expression of cell surface carbohydrate antigens, present in both glycolipids and glycoproteins. These antigens disappear upon differentiation and are replaced with other antigens. We have used the inhibitor of glucosyl ceramide synthetase, 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), to study the distribution of carbohydrate epitopes between glycolipids and glycoproteins. PDMP inhibited the expression of glycolipid antigens, but not glycoprotein antigens assayed by immunofluorescence and thin layer chromatography. In the case of SSEA1, we observed expression on both glycolipids and glycoproteins. Resistance to PDMP inhibition suggests that glycoproteins carry the immunodominant form of SSEA1 on the cell surface of differentiated human EC cells and undifferentiated murine EC cells.