U1A inhibits cleavage at the immunoglobulin m heavy-chain secretory poly(A) site by binding between the two downstream GU-rich regions
U1A inhibits cleavage at the immunoglobulin m heavy-chain secretory poly(A) site by binding between the two downstream GU-rich regions
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DOI：
10.1128/mcb.24.14.6162-6171.2004
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发表时间：
2004-07-01
影响因子：
5.3
通讯作者：
Gunderson, SI
中科院分区：
文献类型：
--
作者：
Phillips, C;Pachikara, N;Gunderson, SI
免疫球蛋白M重链基因座含有两个poly（A）位点，其在B细胞分化期间交替表达。尽管其启动子近端的位置，分泌聚（A）的网站是不表达在未分化的细胞。在B细胞分化过程中，对分泌性poly（A）位点的激活至关重要的是裂解刺激因子64 K与poly（A）位点下游富含GU的元件结合的变化。是什么调节了这种变化还不清楚。分泌性多聚腺苷酸位点含有两个下游富含谷氨酰胺的区域，由29个核苷酸的序列隔开。这两个富含GU的区域对于特异性切割-聚腺苷酸化复合物的结合是必需的。我们在这里证明，U1 A结合两个（AUGCN（1-3）C）基序内的29个核苷酸的序列，并抑制切割刺激因子64 K的结合和切割的分泌聚（A）的网站。
The immunoglobulin M heavy-chain locus contains two poly(A) sites which are alternatively expressed during B-cell differentiation. Despite its promoter proximal location, the secretory poly(A) site is not expressed in undifferentiated cells. Crucial to the activation of the secretory poly(A) site during B-cell differentiation are changes in the binding of cleavage stimulatory factor 64K to GU-rich elements downstream of the poly(A) site. What regulates this change is not understood. The secretory poly(A) site contains two downstream GU-rich regions separated by a 29-nucleotide sequence. Both GU-rich regions are necessary for binding of the specific cleavage-polyadenylation complex. We demonstrate here that U1A binds two (AUGCN(1-3)C) motifs within the 29-nucleotide sequence and inhibits the binding of cleavage stimulatory factor 64K and cleavage at the secretory poly(A) site.