Analysis of phosphatidylethanolamine, phosphatidylcholine, and plasmalogen molecular species in food lipids using an improved 2D high-performance liquid chromatography system
Analysis of phosphatidylethanolamine, phosphatidylcholine, and plasmalogen molecular species in food lipids using an improved 2D high-performance liquid chromatography system
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DOI：
10.1016/j.jchromb.2018.01.014
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发表时间：
2018-03-01
影响因子：
3
通讯作者：
Kitamura, Shinichi
中科院分区：
文献类型：
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作者：
Takahashi, Ryosuke;Nakaya, Makoto;Kitamura, Shinichi
磷脂是细胞膜和食物中一类重要的脂质。已经开发了几种高效液相色谱（HPLC）方法来分析磷脂的分子种类水平。我们开发了一个二维高效液相色谱系统与荷电气溶胶检测器和质谱（MS）分析磷脂酰乙醇胺（PE），磷脂酰胆碱（PC），和他们的缩醛磷脂（PLS）提取的食品材料。因此，即使使用较小的样品，也可以在一个步骤中分析磷脂分子种类。我们证实，大豆卵磷脂的色谱峰大多是基线分离、分配和定量的（PE有24种分子，PC有27种分子）。此外，证实了从鸡胸肉中提取的脂质的色谱图包括缩醛磷脂峰。从鸡胸肉中提取的脂质中的PE部分含有17种乙醇胺缩醛磷脂，相当于总重量的约57%。PC级分仅含有四种胆碱缩醛磷脂，相当于总重量的约11%。pls-PC分子种类的组成不同于pls-PE的组成。在聚乙烯的sn-2位连接的多不饱和脂肪酸由20.5%的20：4脂肪酸组成，并且与sn-1位的碳链无关。然而，在sn-2位的18：1脂肪酸依赖于在sn-1位的碳链。
Phospholipids are an important class of lipids in cell membranes and food. Several high-performance liquid chromatography (HPLC) methods have been developed to analyze phospholipids at the molecular species level. We developed a two-dimensional HPLC system with a charged aerosol detector and mass spectrometry (MS) to analyze phosphatidylethanolamine (PE), phosphatidylcholine (PC), and their plasmalogens (pls) extracted from food materials. Accordingly, the phospholipid molecular species can be analyzed in a single step despite using smaller samples. We confirmed that chromatogram peaks from soybean lecithin are mostly baseline separated, assigned, and quantified (24 molecular species for PE and 27 for PC). In addition, it was confirmed that chromatograms of lipids extracted from chicken breast meat include plasmalogen peaks. The PE fraction in lipids extracted from chicken breast meat contained 17 types of ethanolamine plasmalogens, corresponding to approximately 57% of the total by weight. The PC fraction contained only four choline plasmalogens, corresponding to approximately 11% of the total weight. The composition of the pls-PC molecular species differed from that of pls-PEs. The polyunsaturated fatty acids connected at the sn-2 positions of the pls-PEs consisted of 20.5% 20:4 fatty acid and were independent of the carbon chain at the sn-1 position. However, the 18:1 fatty acid at the sn-2 position was dependent on the carbon chain at the sn-1 position.