The Magnaporthe oryzae Alt A 1-like protein MoHrip1 binds to the plant plasma membrane.
The Magnaporthe oryzae Alt A 1-like protein MoHrip1 binds to the plant plasma membrane.
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DOI：
10.1016/j.bbrc.2017.08.039
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发表时间：
2017-10
影响因子：
3.1
通讯作者：
Yi Zhang;Yingbo Liang;Yijie Dong;Yu-han Gao;Xiufen Yang;Jing-jing Yuan;D. Qiu
中科院分区：
文献类型：
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作者：
Yi Zhang;Yingbo Liang;Yijie Dong;Yu-han Gao;Xiufen Yang;Jing-jing Yuan;D. Qiu
MoHrip 1是从稻瘟病菌中分离到的一种蛋白，属于Alt A 1（AA 1）家族，在水稻侵染过程中，mohrip 1 mRNA水平呈可诱导表达。为了确定MoHrip 1在分枝杆菌中的位置，产生了amohrip 1-gfp突变体。荧光显微镜观察和蛋白质印迹分析表明，MoHrip 1是既存在于分泌组和丰富的真菌细胞壁。为了获得MoHrip 1蛋白，我们在毕赤酵母中进行了MoHrip 1的高效表达。用MoHrip 1处理烟草植株诱导了坏死的形成、活性氧的积累和几种防御相关基因的表达，并赋予了抗病性。通过与绿色荧光蛋白融合，我们发现MoHrip 1能够与烟草和水稻植物质膜结合，在细胞水平上引起快速的形态学变化，如细胞收缩和叶绿体解体。这些发现表明MoHrip 1是一种与微生物相关的分子模式，可被植物免疫系统感知。这是第一个研究的AA 1家族蛋白，可以结合到植物质膜。
MoHrip1, a protein isolated fromMagnaporthe oryzae, belongs to the Alt A 1 (AA1) family.mohrip1mRNA levels showed inducible expression throughout the infection process in rice. To determine the location of MoHrip1 inM. oryzae, amohrip1-gfpmutant was generated. Fluorescence microscopy observations and western blotting analysis showed that MoHrip1 was both present in the secretome and abundant in the fungal cell wall. To obtain MoHrip1 protein, we carried out high-yield expression of MoHrip1 inPichia pastoris. Treatment of tobacco plants with MoHrip1 induced the formation of necrosis, accumulation of reactive oxygen species and expression of several defense-related genes, as well as conferred disease resistance. By fusion to green fluorescent protein, we showed that MoHrip1 was able to bind to the tobacco and rice plant plasma membrane, causing rapid morphological changes at the cellular level, such as cell shrinkage and chloroplast disorganization. These findings indicate that MoHrip1 is a microbe-associated molecular pattern that is perceived by the plant immune system. This is the first study on an AA1 family protein that can bind to the plant plasma membrane.