Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies.
Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies.
复制标题
用于感染性角膜炎的抗菌蓝光疗法：体内和体内研究。
DOI：
10.1167/iovs.16-20272
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发表时间：
2017-01-01
影响因子：
4.4
通讯作者：
Dai T
中科院分区：
文献类型：
--
作者：
Zhu H;Kochevar IE;Behlau I;Zhao J;Wang F;Wang Y;Sun X;Hamblin MR;Dai T
To investigate the effectiveness of antimicrobial blue light (aBL) as an alternative or adjunctive therapeutic for infectious keratitis. We developed an ex vivo rabbit model and an in vivo mouse model of infectious keratitis. A bioluminescent strain of Pseudomonas aeruginosa was used as the causative pathogen, allowing noninvasive monitoring of the extent of infection in real time via bioluminescence imaging. Quantitation of bacterial luminescence was correlated to colony-forming units (CFU). Using the ex vivo and in vivo models, the effectiveness of aBL (415 nm) for the treatment of keratitis was evaluated as a function of radiant exposure when aBL was delivered at 6 or 24 hours after bacterial inoculation. The aBL exposures calculated to reach the retina were compared to the American National Standards Institute standards to estimate aBL retinal safety. Pseudomonas aeruginosa keratitis fully developed in both the ex vivo and in vivo models at 24 hours post inoculation. Bacterial luminescence in the infected corneas correlated linearly to CFU (R2 = 0.921). Bacterial burden in the infected corneas was rapidly and significantly reduced (>2-log10) both ex vivo and in vivo after a single exposure of aBL. Recurrence of infection was observed in the aBL-treated mice at 24 hours after aBL exposure. The aBL toxicity to the retina is largely dependent on the aBL transmission of the cornea. Antimicrobial blue light is a potential alternative or adjunctive therapeutic for infectious keratitis. Further studies of corneal and retinal safety using large animal models, in which the ocular anatomies are similar to that of humans, are warranted.
