The variable domain from dynamin-related protein 1 promotes liquid-liquid phase separation that enhances its interaction with cardiolipin-containing membranes.
The variable domain from dynamin-related protein 1 promotes liquid-liquid phase separation that enhances its interaction with cardiolipin-containing membranes.
复制标题
动力相关蛋白 1 的可变结构域可促进液-液相分离，从而增强其与含心磷脂膜的相互作用。
DOI：
10.1002/pro.4787
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Hill,RBlake
中科院分区：
文献类型：
--
作者：
Posey,AmmonE;Ross,KyleA;Bagheri,Mehran;Lanum,ElizabethN;Khan,MishaA;Jennings,ChristineE;Harwig,MeganC;Kennedy,NolanW;Hilser,VincentJ;Harden,JamesL;Hill,RBlake
Dynamins are an essential superfamily of mechanoenzymes that remodel membranes and often contain a “variable domain” important for regulation. For the mitochondrial fission dynamin, dynamin‐related protein 1, a regulatory role for the variable domain (VD) is demonstrated by gain‐ and loss‐of‐function mutations, yet the basis for this is unclear. Here, the isolated VD is shown to be intrinsically disordered and undergo a cooperative transition in the stabilizing osmolyte trimethylamine N‐oxide. However, the osmolyte‐induced state is not folded and surprisingly appears as a condensed state. Other co‐solutes including known molecular crowder Ficoll PM 70, also induce a condensed state. Fluorescence recovery after photobleaching experiments reveal this state to be liquid‐like indicating the VD undergoes a liquid–liquid phase separation under crowding conditions. These crowding conditions also enhance binding to cardiolipin, a mitochondrial lipid, which appears to promote phase separation. Since dynamin‐related protein 1 is found assembled into discrete punctate structures on the mitochondrial surface, the inference from the present work is that these structures might arise from a condensed state involving the VD that may enable rapid tuning of mechanoenzyme assembly necessary for fission.
