The first DNA 1-like α satellites in association with New World begomoviruses in natural infections
The first DNA 1-like α satellites in association with New World begomoviruses in natural infections
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DOI：
10.1016/j.virol.2010.05.003
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发表时间：
2010-09-01
期刊：
影响因子：
3.7
通讯作者：
Jeske, H.
中科院分区：
文献类型：
--
作者：
Paprotka, T.;Metzler, V.;Jeske, H.
从巴西杂草双生病毒感染的典型症状，DNAs的两个新的病毒种，两个新的菌株与两个变种的已知的双分Begomoviruses进行测序。此外，在新大陆自然存在的前两个DNA 1样卫星（α卫星）也被发现。它们与纳米病毒DNA组分相关，并显示出典型的基因组组织，具有一个可能编码复制相关蛋白（Rep）的开放阅读框架、保守的发夹结构和富含A的区域。在与它们的辅助菜豆花叶病毒（Euphorbia mosaic virus，EuMV）或醉蝶花叶皱曲病毒（Cleome leaf crumple virus，ClLCrV）共接种后，卫星DNA被传递到实验和自然寄主植物中。三个菜豆花叶病毒分离株（EuMV和ClLCrV）感染拟南芥植物，诱导轻微的症状，其中之一（ClLCrV）transreplicated卫星有效。因此，这一重要的模式植物的分子分析提供了几个新的工具。(C)2010年爱思唯尔公司All rights reserved.
From Brazilian weeds with typical symptoms of a geminivirus infection, the DNAs of two new virus species, two new strains with two variants of already known bipartite begomoviruses were sequenced. Moreover, the first two DNA 1-like satellites (alpha satellites) occurring naturally in the New World were identified. They are related to nanoviral DNA components and show a typical genome organization with one open reading frame coding potentially for a replication-associated protein (Rep), a conserved hairpin structure, and an A-rich region. After coinoculation with their helper begomoviruses (Euphorbia mosaic virus, EuMV or Cleome leaf crumple virus, ClLCrV) the satellite DNAs were transmitted to experimental and natural host plants. Three of the begomovirus isolates (EuMV and ClLCrV) infected Arabidopsis thaliana plants, induced mild symptoms, and one of these (ClLCrV) transreplicated the satellite efficiently. As a result, several novel tools for molecular analyses of this important model plant are provided. (C) 2010 Elsevier Inc. All rights reserved.