miR-15a and miR-16-1 down-regulation in pituitary adenomas
miR-15a and miR-16-1 down-regulation in pituitary adenomas
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DOI：
10.1002/jcp.20282
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发表时间：
2005-07-01
影响因子：
5.6
通讯作者：
Uberti, ECD
中科院分区：
文献类型：
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作者：
Bottoni, A;Piccin, D;Uberti, ECD
微小RNA（miRs）是小型非编码RNA，作为其他RNA的反义调节因子发挥作用。miR - 15a和miR - 16 - 1基因位于13q14号染色体，这是垂体肿瘤中经常缺失的一个区域。在B细胞慢性淋巴细胞白血病（B - CLL）中，miR - 15a和miR - 16 - 1的表达与精氨酰 - tRNA合成酶（RARS）的表达水平呈负相关，RARS是一种在氨酰 - tRNA合成酶复合物中与辅因子p43相关联的酶。当p43被分泌时，它可调节局部炎症反应和巨噬细胞趋化性，并且在小鼠中似乎具有抗肿瘤特性。我们通过Northern杂交检测了10例生长激素（GH）分泌型和10例催乳素（PRL）分泌型垂体大腺瘤中miR - 15a和miR - 16 - 1的表达，并研究了其与体内外特征可能存在的相关性。我们发现，与正常垂体组织相比，垂体腺瘤中miR - 15a和miR - 16 - 1的表达水平较低。此外，它们的表达与肿瘤直径以及RARS的表达呈负相关（P
Micro RNAs (miRs) are small noncoding RNAs, functioning as antisense regulators of other RNAs. miR-15a and miR-16-1 genes are located at chromosome 13q14, a region which is frequently deleted in pituitary tumors. An inverse correlation has been shown in B cell chronic lymphocytic leukemia (B-CLL) between miR-15a and miR-16-1 expression and the expression levels of arginyl-tRNA synthetase (RARS), an enzyme which associates with the cofactor p43 in the aminoacyl-tRNA synthetase complex. When secreted, p43 regulates local inflammatory response and macrophage chemotaxis, and seems to have anti-neoplastic properties in mice. We explored miR-15a and miR-16-1 expression in 10 GH-secreting and in 10 PRL-secreting pituitary macroadenomas by Northern blot, and investigated the possible correlation with in vivo and in vitro characteristics. We found that miR-15a and miR-16-1 are expressed at lower levels in pituitary adenomas as compared to normal pituitary tissue. Moreover, their expression inversely correlates with tumor diameter and with RARS expression (P