Labelling plants in the Chernobyl way: A new 137Cs and 14C foliar application approach to investigate rhizodeposition and biopore reuse
Labelling plants in the Chernobyl way: A new 137Cs and 14C foliar application approach to investigate rhizodeposition and biopore reuse
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以切尔诺贝利方式标记植物：一种新的 137Cs 和 14C 叶面施用方法，用于研究根际沉积和生物孔再利用
DOI：
10.1007/s11104-017-3260-7
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发表时间：
2017
期刊：
影响因子：
4.9
通讯作者：
Kuzyakov
中科院分区：
文献类型：
--
作者：
Banfield;Zarebanadkouki;Kuzyakov
背景和目的生物孔作为微生物热点为作物提供额外的营养——但前提是它们的根在生物孔内生长。这种重复利用从未被量化，因为作物前特异性生物孔与大量预先存在的生物孔几乎没有区别。量化需要对作物前（137Cs，标记其生物孔）和主要作物（14C，检测新根）进行放射性核素标记。对模拟生物孔再利用进行了初步试验：给予同一株植物的两种核素排泄到同一根际。方法紫花苜蓿（cv. p . a .）和紫花苜蓿（cv. b . a .）；每颗行星的叶子上都有137cs和14co2的顺序标记。β-信号通过水平土壤切割的成像被可视化——有或没有屏蔽弱的14c。结果两种植物分别分配了7.1% ~ 9.4%的地下碳和21.63%的地下碳。第一张图给出了两种活动；而第二组只给出了137c。从第一幅图像中减去第二幅图像得到14c分布，从而成功地分离了信号。因此，获得了根的单独空间表示。137cs和14c的主根位置空间重叠系数非常高（> 0.95）。结论采用这种顺序标记和屏蔽方法进行生物孔再利用定量分析是可行的。
Background and aimsBiopores as microbial hotspots provide additional nutrients to crops – but only if their roots grow within the biopores. Such reuse has never been quantified as pre-crop-specific biopores are hardly differentiated from the multitude of pre-existing biopores. Quantification requires e.g. radionuclide labelling of pre-crops (137Cs, to label their biopores) and main crops (14C, to detect new roots). Preliminary testing was performed on simulated biopore reuse: both nuclides given to the same plant were excreted into the same rhizosphere.MethodsCichorium intybus (cv.Puna) andMedicago sativa(cv. Planet) were each sequentially labelled via the leaves with137Cs and14CO2. β-signals were visualised by imaging of horizontal soil cuts - with and without shielding off the weaker14C.ResultsBoth species allocated 7.1–9.4% of the137Cs and 21–63% of the14C below ground. The first image gave both activities; while the second gave only137Cs. Subtracting the second from the first image gave the14C distribution, resulting in successful separation of the signals. Thus, separate spatial representations of the roots were obtained. Main root locations by137Cs and14C showed a very high spatial overlap coefficient (> 0.95).ConclusionsBiopore reuse quantification likely becomes feasible with this sequential labelling and shielding approach.
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