Hepatitis B virus genotype G is an extremely rare genotype in Japan
Hepatitis B virus genotype G is an extremely rare genotype in Japan
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DOI：
10.1016/j.hepres.2004.09.004
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发表时间：
2004-12-01
影响因子：
4.2
通讯作者：
Mizokami, M
中科院分区：
文献类型：
--
作者：
Kato, H;Sugauchi, F;Mizokami, M
背景：乙肝病毒(乙肝病毒)分为A-G等7种基因型。HBV型在地理分布上有一定的特点。由于HBVG基因(HBVG)在日本的发现较晚，对其在日本的分布情况知之甚少。本研究的目的是为了澄清这一问题。患者和方法：721份来自日本乙肝患者的血清样本被调查。这些患者包括149名无症状携带者，325名慢性肝炎患者，129名肝硬变患者和118名肝细胞癌患者。针对乙肝病毒基因组S区，采用限制性内切酶片段长度多态性方法，确定了6种不同的基因型(A-F)。结果：在所调查的721例血清标本中，有12例为HBVA型，88例为HBVB型，610例为HBVC型，3例为HBVD型，1例为HBVF型。7例患者存在不同的混合感染类型，其中2例为HBVA型和HBVD型，5例为HBVB型和HBVC型。在日本，乙肝病毒/G似乎是一种极其罕见的基因型别。(C)2004爱思唯尔B.V.保留所有权利。
Background: Hepatitis B virus (HBV) has been classified into seven genotypes (A-G). HBV genotypes have a geographically characteristic distribution. Since HBV genotype G (HBV/G) was identified recently, little is known about the distribution of HBV/G in Japan. The aim of this study was to clarify this issue.Patients and methods: Seven hundred and twenty-one serum samples obtained from patients with HBV in Japan were investigated. The patients included 149 asymptomatic carriers, 325 with chronic hepatitis, 129 with liver cirrhosis, and 118 with hepatocellular carcinoma. Six HBV genotypes (A-F) were determined by restriction fragment length polymorphim targeting to the S region of the HBV genome. Furthermore, HBV/G was investigated by polymerase chain reaction with hemi-nested primers derived from an HBV/G-specific nucleotide sequence.Results: Of the 721 serum samples investigated, 12 subjects were classified as having HBV/A, 88 HBV/B, 610 HBV/C, 3 HBV/D, and 1 HBV/F. Seven subjects had a mixed infection with distinct genotypes, two with HBV/A and HBV/D, and five with HBV/B and HBV/C. HBV/G was not identified among the 721 samples.Conclusion: HBV/G was not identified in a large cohort of patients with HBV, either single or dual infection. HBV/G seems to be an extremely rare genotype in Japan. (C) 2004 Elsevier B.V. All rights reserved.