Purkinje cells loss in off spring due to maternal morphine sulfate exposure: a morphometric study.
Purkinje cells loss in off spring due to maternal morphine sulfate exposure: a morphometric study.
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DOI：
10.5115/acb.2012.45.2.121
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发表时间：
2012-06
影响因子：
1.1
通讯作者：
Ghafari S
中科院分区：
文献类型：
--
作者：
Golalipour MJ;Ghafari S
研究了硫酸吗啡对成人大脑皮层和新生一日新生儿小脑的毒性作用。本研究旨在探讨妊娠期和哺乳期母体吗啡对18日龄和32日龄小鼠子代浦肯野细胞和小脑皮质层的影响。30只雌性小鼠被随机分为实验组和对照组。在交配前7天、妊娠期（GD 0-21） 18天或32天腹腔注射硫酸吗啡（10 mg/kg/体重）。对照组接受等量生理盐水。每组取6例婴儿小脑，分别用甲酚紫染色。对小脑皮层进行定量计算机辅助形态学研究。2个实验组（出生日后[P]18, 23.40±0.5;P32, 23.45±1.4）的线性浦肯野细胞密度均较对照组（P18, 28.70±0.9;P32, 28.95±0.4）显著降低（P<0.05）。试验组浦肯野细胞面积、单叶顶端周长、直径和深度均显著低于对照组（P<0.05）。吗啡治疗组大鼠小脑皮层浦肯野层厚度明显减少（P<0.05）。本研究表明，在怀孕前、怀孕期间和哺乳期给药吗啡可导致18日龄和32日龄幼鼠浦肯野细胞丢失和浦肯野细胞大小减小。
The toxic effects of morphine sulfate in the adult cerebral cortex and one-day neonatal cerebellum have been studied. This study was carried out to evaluate the effect of maternal morphine exposure during gestational and lactation period on the Purkinje cells and cerebellar cortical layer in 18- and 32-day-old mice offspring. Thirty female mice were randomly allocated into cases and controls. In cases, animals received morphine sulfate (10 mg/kg/body weight intraperitoneally) during the 7 days before mating, gestational day (GD 0-21) 18 or 32. The controls received an equivalent volume of saline. The cerebellum of six infants for each group was removed and each was stained with cresyl violet. Quantitative computer-assisted morphometric study was done on cerebellar cortex. The linear Purkinje cell density in both experimental groups (postnatal day [P]18, 23.40±0.5; P32, 23.45±1.4) were significantly reduced in comparison with the control groups (P18, 28.70±0.9; P32, 28.95±0.4) (P<0.05). Purkinje cell area, perimeter and diameter at apex and depth of simple lobules in the experimental groups were significantly reduced compared to the controls (P<0.05). The thickness of the Purkinje layer of the cerebellar cortex was significantly reduced in morphine treated groups (P<0.05). This study reveals that morphine administration before pregnancy, during pregnancy and during the lactation period causes Purkinje cells loss and Purkinje cell size reduction in 18- and 32-day-old infant mice.