Carbon Monoxide Is Involved in Hydrogen Gas-Induced Adventitious Root Development in Cucumber under Simulated Drought Stress.
Carbon Monoxide Is Involved in Hydrogen Gas-Induced Adventitious Root Development in Cucumber under Simulated Drought Stress.
复制标题
一氧化碳参与模拟干旱胁迫下氢气诱导的黄瓜不定根发育
DOI：
10.3389/fpls.2017.00128
复制
发表时间：
2017
影响因子：
5.6
通讯作者：
Li C
中科院分区：
文献类型：
--
作者：
Chen Y;Wang M;Hu L;Liao W;Dawuda MM;Li C
氢气（H2）和一氧化碳（CO）参与植物的生长和发育过程，并可诱导植物对多种胁迫的耐受性。然而，干旱条件下H2和CO在不定根发育中的独立作用和互作效应尚未得到应有的研究关注。我们推测在干旱胁迫下不定根的发育过程中H2和CO之间存在着相互作用。结果表明，50%（v/v）的富氢水（HRW）、500 μM氯化血红素（Hemin）和30%（w/v）的CO水溶液对黄瓜外植体不定根的发生有明显的促进作用。在干旱胁迫下。H2和CO增加了干旱条件下叶片的相对含水量（RWC）、叶绿素含量（叶绿素a、B和a+B）和叶绿素荧光参数（光系统II的光化学效率（PSII）、PSII实际光化学效率和光化学猝灭系数）。当一氧化碳清除剂血红蛋白（Hb）或锌原卟啉IX（ZnPPIX）加入到HRW/CO水溶液中时，HRW/CO水溶液对RWC、叶片叶绿素含量和叶绿素荧光参数的正效应被逆转。此外，超氧化物歧化酶，过氧化物酶，过氧化氢酶，抗坏血酸过氧化物酶显着增加，在外植体与HRW和CO水溶液在干旱胁迫下处理，从而减轻氧化损伤，减少硫代巴比妥酸反应物质（TBARS），过氧化氢（H2 O2），和超氧自由基（O2-）水平。H2和CO还提高了可溶性糖、可溶性总蛋白和脯氨酸含量。然而，上述CO/H2介导的效应被CO清除剂Hb或CO特异性合成抑制剂ZnPPIX逆转。因此，CO可能通过提高RWC、叶片叶绿素含量、叶绿素荧光参数、代谢成分含量、激活抗氧化酶、降低TBARS、O2-和H2 O2水平，参与了干旱胁迫下H2诱导的不定根形成，减轻了氧化损伤。
Hydrogen gas (H2) and carbon monoxide (CO) are involved in plant growth and developmental processes and may induce plant tolerance to several stresses. However, the independent roles and interaction effect of H2 and CO in adventitious root development under drought conditions have still not received the needed research attention. We hypothesize that there exists crosstalk between H2 and CO during adventitious root development under drought stress. The results of our current study revealed that 50% (v/v) hydrogen-rich water (HRW), 500 μM Hemin (the CO donor) and 30% (w/v) CO aqueous solution apparently promoted the development of adventitious roots in cucumber explants (Cucumis Sativus L.) under drought stress. H2 and CO increased relative water content (RWC), leaf chlorophyll content (chlorophyll a, b, and a+b), and chlorophyll fluorescence parameters [photochemical efficiency of photosystem II (PSII), PSII actual photochemical efficiency and photochemical quench coefficient] under drought condition. When the CO scavenger hemoglobin (Hb) or zinc protoporphyrin IX (ZnPPIX) was added to HRW/CO aqueous solution, the positive effect of HRW/CO aqueous solution on RWC, leaf chlorophyll content, and chlorophyll fluorescence parameters were reversed. Additionally, superoxide dismutases, peroxidase, catalase, and ascorbate peroxidase was significantly increased in the explants treated with HRW and CO aqueous solution under drought stress, thus alleviating oxidative damage, as indicated by decreases in thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H2O2), and superoxide radical (O2-) levels. H2 and CO also improved the levels of water soluble carbohydrate, total soluble protein, and proline content. However, the above CO/H2-mediated effects were reversed by CO scavenger Hb or CO specific synthetic inhibitor ZnPPIX. Therefore, CO may be involved in H2-induced adventitious rooting under drought stress and alleviate oxidative damage by enhancing RWC, leaf chlorophyll content, chlorophyll fluorescence parameters, metabolic constituent content, activating anti-oxidant enzymes and reducing TBARS, O2-, and H2O2 levels.