Zebrafish xenografts as a tool for in vivo studies on human cancer
Zebrafish xenografts as a tool for in vivo studies on human cancer
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DOI：
10.1111/j.1749-6632.2012.06575.x
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Lengerke, Claudia
中科院分区：
文献类型：
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作者：
Konantz, Martina;Balci, Tugce B.;Lengerke, Claudia
斑马鱼已经成为胚胎发育和器官发生的遗传研究以及越来越多的癌症生物学研究的强有力的脊椎动物模型。斑马鱼促进反向和正向遗传方法的执行，包括诱变和小分子筛选。此外，一些研究报告了将人类细胞异种移植到斑马鱼胚胎和成鱼体内的可行性。该模型提供了一个独特的机会来监测肿瘤诱导的血管生成、侵袭性和对一系列体内和实时治疗的反应。尽管鱼类和人类之间的基因功能高度保守，但人们仍然担心斑马鱼组织生态位的潜在差异和/或缺失的微环境线索可能会限制斑马鱼人类癌细胞异种移植模型数据的相关性和转化效用。在这里，我们总结了目前关于人类细胞异种移植到斑马鱼体内的数据，强调了该模型与经典小鼠异种移植模型相比的优势和局限性。
The zebrafish has become a powerful vertebrate model for genetic studies of embryonic development and organogenesis and increasingly for studies in cancer biology. Zebrafish facilitate the performance of reverse and forward genetic approaches, including mutagenesis and small molecule screens. Moreover, several studies report the feasibility of xenotransplanting human cells into zebrafish embryos and adult fish. This model provides a unique opportunity to monitor tumor-induced angiogenesis, invasiveness, and response to a range of treatments in vivo and in real time. Despite the high conservation of gene function between fish and humans, concern remains that potential differences in zebrafish tissue niches and/or missing microenvironmental cues could limit the relevance and translational utility of data obtained from zebrafish human cancer cell xenograft models. Here, we summarize current data on xenotransplantation of human cells into zebrafish, highlighting the advantages and limitations of this model in comparison to classical murine models of xenotransplantation.