Fifteen years of successful spread of Salmonella enterica serovar Mbandaka clone ST413 in Poland and its public health consequences
Fifteen years of successful spread of Salmonella enterica serovar Mbandaka clone ST413 in Poland and its public health consequences
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DOI：
10.5604/12321966.1203883
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发表时间：
2016-01-01
影响因子：
1.7
通讯作者：
Wasyl, Dariusz
中科院分区：
文献类型：
--
作者：
Hoszowski, Andrzej;Zajac, Magdalena;Wasyl, Dariusz
20世纪90年代，肠道沙门氏菌血清型（S.）姆班达卡在波兰的饲料和家禽中发生。在随后的几年里，该血清型在其他欧盟国家也获得了流行病学的重要性。本研究的目的是评估当代S. Mbandaka与源自流行病开始时的分离株进行比较，并评估家禽作为人类感染源的贡献。70 S主要在2009 - 2010年从人类、家禽、食物和饲料中分离的姆班达卡用API ID 32（R）、MIC、质粒谱、PFGE和MLST进行分型。采用PCR和测序方法鉴定质粒介导的喹诺酮类和头孢菌素类耐药机制。共鉴定出6个生化谱型，59个S.姆班达卡被证明对使用的抗菌剂敏感。8株菌携带质粒，其中少数菌株bla（CMY-2）和qnrS 1基因阳性。结果发现，15个XbaI-PFGE图谱有两个聚类，相似性为77.5%，第一个聚类包括7个历史分离株和几个当代非人沙门氏菌的XbaI-PFGE图谱;姆班达卡第二个聚类中的主要特征包括28个人和1个肉鸡分离株。MLST分析表明，序列类型ST 413发生在所有测试的菌株。鉴定了S.人和家禽来源的姆班达卡表明动物是人类感染的主要途径。尽管沙门氏菌控制计划，S。Mbandaka ST 413克隆已经在波兰流行了好几年。食物生产链中的沙门氏菌控制政策应不断更新，以针对具有重要流行病学意义的血清型。在S.姆班达卡对氟喹诺酮类和头孢菌素类等抗菌药物的滥用可能会妨碍公共卫生。
In the 1990s, Salmonella enterica serovar (S.) Mbandaka occurred in feed and poultry in Poland. In the following years, the serovar also gained epidemiological importance in other EU countries. The objectives of current study were to evaluate the genetic relationship of contemporary S. Mbandaka with isolates originating from the beginning of the epidemics, and to assess the contribution of poultry as the source of infections in humans. Seventy S. Mbandaka isolated mainly in 2009 - 2010 from humans, poultry, food, and feed were typed with API ID32 (R), MIC, plasmid profiling, PFGE, and MLST. PCR and sequencing were used to identify plasmid mediated quinolone and cephalosporin resistance mechanisms. Six biochemical profiles were identified and 59 of S. Mbandaka proved to be susceptible to the applied antimicrobials. Eight strains carried plasmids and a few of them were positive for bla(CMY-2) and qnrS1 genes. Two clusters of 15 XbaI-PFGE profiles with similarity of 77.5% were found. The first cluster, gathered 7 profiles involving historical isolates and several contemporary non-human S. Mbandaka. The predominant profile in the second cluster consisted of 28 human and 1 broiler isolate. MLST analysis showed sequence type ST413 occurring among all tested isolates. The identification of close genetic relationships between S. Mbandaka of human and poultry origin indicates animals as a primal human infection route. Despite Salmonella control programmes, the S. Mbandaka ST413 clone has been circulating for several years in Poland. Salmonella control polices in food production chain should be continuously updated to target serovars of major epidemiological importance. Resistance noted in S. Mbandaka to such antimicrobials as fluoroquinolones and cephalosporins may hinder public health.