A role for chromatin remodeling in regulation of CUC gene expression in the Arabidopsis cotyledon boundary
A role for chromatin remodeling in regulation of CUC gene expression in the Arabidopsis cotyledon boundary
复制标题
DOI：
10.1242/dev.02508
复制
发表时间：
2006-08-15
期刊：
影响因子：
4.6
通讯作者：
Wagner, Doris
中科院分区：
文献类型：
--
作者：
Kwon, Chang Seob;Hibara, Ken-Ichiro;Wagner, Doris
杯形子叶（CUC）基因CUC 1、CUC 2和CUC 3在拟南芥中冗余地控制子叶分离。为了鉴定这一过程的新调节剂，我们使用cuc 2的无效等位基因cuc 2 -1进行了表型增强子筛选。我们鉴定了AtBRM的三个无义等位基因，AtBRM是一种拟南芥SWI/SNF染色质重塑ATP酶，在cuc 2 - 1中导致强烈的子叶融合。atbrm也增强了功能丧失的cuc 1和cuc 3突变体的子叶融合，这表明在子叶分离中普遍需要这种ATP酶。相比之下，一个无效等位基因的SPLAYED（SYD），在拟南芥AtBRM的最接近的同源物，增强功能丧失的cuc 1突变体。通过调查活动的CUC启动子在子叶边界在致敏背景下的胚胎发生过程中，我们表明，AtBRM上调所有三个CUC基因的转录，而SYD上调CUC 2的表达。我们的研究结果揭示了一个特定的作用，染色质重塑ATP酶在胚胎发生过程中的边界细胞的形成和/或维护。
The CUP-SHAPED COTYLEDON (CUC) genes CUC1, CUC2 and CUC3 act redundantly to control cotyledon separation in Arabidopsis. In order to identify novel regulators of this process, we have performed a phenotypical enhancer screen using a null allele of cuc2, cuc2-1. We identified three nonsense alleles of AtBRM, an Arabidopsis SWI/SNF chromatin remodeling ATPase, that result in strong cotyledon fusion in cuc2-1.atbrm also enhances cotyledon fusion in loss-of-function cuc1 and cuc3 mutants, suggesting a general requirement for this ATPase in cotyledon separation. By contrast, a null allele of SPLAYED (SYD), the closest homolog of AtBRM in Arabidopsis, enhances only the loss-of-function cuc1 mutant. By investigating the activities of the CUC promoters in the cotyledon boundary during embryogenesis in sensitized backgrounds, we demonstrate that AtBRM upregulates the transcription of all three CUC genes, whereas SYD upregulates the expression of CUC2. Our results uncover a specific role for both chromatin remodeling ATPases in the formation and/or maintenance of boundary cells during embryogenesis.