Long-term history dependence of growth rates of E. coli after nutrient shifts.
Long-term history dependence of growth rates of E. coli after nutrient shifts.
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营养物质变化后大肠杆菌生长率的长期历史依赖性。
DOI：
10.1101/2023.08.22.554350
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Basan,Markus
中科院分区：
文献类型：
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作者：
Christodoulou,Dimitris;Mukherjee,Avik;Wegmann,Rebekka;Pagano,Adriano;Sharma,Varun;Linker,StephanieMaria;Chang,Yu-Fang;Palme,JuliusSebastian;Sauer,Uwe;Basan,Markus
根据一个被广泛接受的微生物学范式，稳态增长率仅由当前的增长条件1 -3和生长状态之间的适应是迅速的，最近概括了简单的资源分配模型4。然而，即使在微生物中，重叠的调控网络也可以产生多稳定性或长期细胞记忆。像单核细胞增生李斯特菌和枯草芽孢杆菌这样的物种“区分”了不同的孢子形成历史，但目前还不清楚这些状态是否可以持续许多代。值得注意的是，在研究大肠杆菌的碳共利用时，我们发现碳源组合的生长速率可能严重依赖于先前的生长条件。在相同的条件下生长，我们观察到高达25%的生长率差异，我们没有观察到超过15代的生长率收敛。我们观察到这种现象发生在不同的磷酸转移酶（PTS）底物与各种致突变碳源的组合中，并发现它依赖于转录因子Mlc。
According to a widely accepted paradigm of microbiology, steady-state growth rates are determined solely by current growth conditions1–3 and adaptations between growth states are rapid, as recently recapitulated by simple resource allocation models4. However, even in microbes overlapping regulatory networks can yield multi-stability or long-term cellular memory. Species like Listeria monocytogenes5 and Bacillus subtilis “distinguish” distinct histories for the commitment to sporulation6, but it is unclear if these states can persist over many generations. Remarkably, studying carbon co-utilization of Escherichia coli, we found that growth rates on combinations of carbon sources can depend critically on the previous growth condition. Growing in identical conditions, we observed differences in growth rates of up to 25% and we did not observe convergence of growth rates over 15 generations. We observed this phenomenon occurs across combinations of different phosphotransferase (PTS) substrates with various gluconeogenic carbon sources and found it to depend on the transcription factor Mlc.