2-D RP/RPLC method to separate components in Fructus schisandrae chinensis.
2-D RP/RPLC method to separate components in Fructus schisandrae chinensis.
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DOI：
10.1002/jssc.200900563
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发表时间：
2010-03
影响因子：
3.1
通讯作者：
Gaowa Jin;Yuntao Dai;Jia-tao Feng;Xuemei Qin;X. Xue;Feifang Zhang;Xinmiao Liang
中科院分区：
文献类型：
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作者：
Gaowa Jin;Yuntao Dai;Jia-tao Feng;Xuemei Qin;X. Xue;Feifang Zhang;Xinmiao Liang
建立了一种离线二维反相/反相高效液相色谱法分离中药北五味子提取物中的成分。该方法采用Click β-CD固定相结合C18柱构建二维RP/RPLC系统。β-CD柱在RP模式下分离中药中的化合物方面显示出巨大的潜力。从用于第一维的Click β-CD柱收集的级分被解析成许多额外的组分，表明基于Click β-CD和C18相的2-D LC系统是正交的。两个色谱柱上的移动的相相同。这种2-D LC方法不仅使我们能够分离一维色谱法无法分辨的组分，而且还可以更有效地检测微量样品组分。
An off-line 2-D RP/RPLC method was developed to separate components in extracts of Fructus schisandrae chinensis, which is a traditional Chinese medicine. In this method, Click beta-CD stationary phase was proposed for constructing 2-D RP/RPLC system combined with C18 column. Beta-CD column showed great potential to separate compounds in traditional Chinese medicines under RP mode. Collected fractions from Click beta-CD column used in first dimension were resolved into many additional components, indicating that the 2-D LC system based on Click beta-CD and C18 phases is orthogonal. The mobile phase was same on both columns. This 2-D LC method facilitates us not only to separate components that cannot be resolved by unidimensional chromatography, but also to achieve much more efficient detection of minor sample components.