Effects of fatty acid from deep-sea microorganisms on lipid bilayer membrane fluidity under high pressure: comparison of branched-chain and polyunsaturated fatty acid
Effects of fatty acid from deep-sea microorganisms on lipid bilayer membrane fluidity under high pressure: comparison of branched-chain and polyunsaturated fatty acid
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深海微生物脂肪酸在高压下对脂质双层膜流动性的影响：支链脂肪酸和多不饱和脂肪酸的比较
DOI：
10.1051/e3sconf/202132201019
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发表时间：
2021
影响因子：
--
通讯作者：
Nishimoto Makoto
中科院分区：
文献类型：
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作者：
Miura Kentaro;Ueno Hiroto.;Numa Yu;Morita Seiichi.;Nishimoto Makoto
用时间分辨荧光各向异性方法研究了高温高压下微生物生物膜中不饱和和饱和支链脂肪酸对生物膜流动性的影响。首先，用三种不同结构的脂类研究了有序参数S和旋转扩散系数D_w与脂分子运动的关系，发现前者与脂分子的间距有关，后者与脂分子的运动有关。接下来，我们研究了含有饱和支链脂肪酸12-甲基十三烷酸(12-MTA)和顺-4，7，10，13，16，19-二十二碳六烯酸(DHA)的多不饱和脂肪酸(PUFA)的脂质双层膜的SandDW值。结果表明，12-MTA增加了S值，降低了Dw值。另一方面，DHA倾向于降低S值和增加Dw值，尽管幅度很小。这些结果表明，12-MTA缩小了脂类的分子间距，抑制了脂类的分子运动，而DHA则倾向于加宽脂类的分子间距，促进脂类的分子运动。
In this study, the effects of unsaturated and saturated branchedchain fatty acids in biomembranes of microorganisms living under high temperature and high pressure on the fluidity of biomembranes were investigated by time-resolved fluorescence anisotropy measurements. First, the relationship between the order parameterSand the rotational diffusion coefficientDw, which can be calculated from the fluorescence anisotropy measurements, and the motion of lipid molecules was investigated using lipids with three different structures, and it was found that the former was related to the spacing of lipid molecules and the latter to the motion of lipid molecules. Next, we investigated theSandDwvalues of lipid bilayer membrane containing the saturated branched-chain fatty acid 12-methyltridecanoic acid (12-MTA) and the polyunsaturated fatty acid (PUFA)cis-4,7,10,13,16,19-docosahexaenoic acid (DHA). The results showed that 12-MTA increased theSvalue and decreased theDwvalue. On the other hand, DHA tended to reduce theSvalue and increase theDwvalue, albeit slightly. These results mean that 12-MTA narrows the molecular spacing of lipids and inhibits lipid molecular movement, while DHA tends to widen the molecular spacing of lipids and promote lipid molecular movement.