Antimicrobial properties and phytochemical composition of Garcinia kola, Bryophyllum pinnatum, and Allium sativum juices on some clinical pathogens.
Antimicrobial properties and phytochemical composition of Garcinia kola, Bryophyllum pinnatum, and Allium sativum juices on some clinical pathogens.
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DOI：
10.4236/abb.2021.1211025
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发表时间：
2021-01-01
期刊：
影响因子：
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通讯作者：
Monsi, Tombari Pius
中科院分区：
文献类型：
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作者：
Amala, Smart Enoch;Nweke, Simeon Nicholas;Monsi, Tombari Pius
背景：自人类起源以来，药用植物就一直在使用。许多重要的化学物质具有生物功能，可用于治疗和预防细菌、真菌、草食哺乳动物和昆虫的攻击，这些物质是由不同的植物产生的。这些具有有用性质的化合物已被记录在案，约有1.2万种，约占植物总种数的10%。目的：研究中草药提取物对临床致病菌的抑菌作用。方法：测定大蒜、羽扇豆汁、藤黄压榨汁及其稀释液对大肠杆菌、肺炎克雷伯菌、奇异变形杆菌、金黄色葡萄球菌、白色念珠菌等病原菌的抑菌活性，确定其对其及其组合的敏感性。采用琼脂扩散法测定米勒-辛顿琼脂的药敏图谱。对植物汁液进行了植物化学分析。结果：在100%(整齐)条件下，葛根汁、鸡血藤果汁和藤黄果汁对病原菌均有明显的抑菌圈，抑菌圈直径在22.0 mm以上。在75%的浓度下，果汁对针对它们的病原体具有抑制作用。大蒜对肺炎克雷伯氏菌、奇异肺炎克雷伯氏菌和金黄色葡萄球菌的抑制率为50%。白色念珠菌对柯拉汁的敏感率为60%，100%浓度时为40%。当果汁浓度为75%时，白色念珠菌分离株对果汁的敏感率也达到60%。在50%~100%浓度范围内，白念珠菌对大蒜提取物的敏感性为100%。结论：药用植物汁液对革兰氏阳性菌、革兰氏阴性菌及真菌具有一定的抗菌潜力。
Background: Medicinal plants have been in use since the origin of man. Many important chemical substances with biological functions that could be used for treatment and prevention of attack from bacteria, fungi, herbivorous mammals and insects are produced by different plants. Such compounds with useful properties have been recorded in their numbers, about 12,000 accounting for about 10% of total plant species. Aim: The aim of the study was to determine the antimicrobial efficacies of herbal extracts on some clinical pathogens. Methods: The antimicrobial activities of pressed juices of Allium sativum (garlic), Bryophyllum pinnatum and Garcinia kola neats and their dilutions were tested on pathogens such as Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus and Candida albicans to determine their susceptibility to the juices and their combinations. Agar well diffusion method was employed on Muller-Hinton agar to determine their antimicrobial susceptibility pattern. The phytochemical analysis of the plants' juices were also determined. Results: At 100% (neat) the juices of G. kola, B. pinnatum and Garcinia kola showed substantial zones of inhibition against the pathogens with a zone diameter of about 22.0 mm and above. At 75% concentrations, the juices inhibited the pathogens tested against them. A. sativum (garlic) inhibited K. pneumonia, P. mirabilis, and S.aureus even at 50% concentration. C. albicans isolates were 60% susceptible to G. kola juice, 40% at 100% concentration. At 75% concentration of the juice, C. albicans isolates were also 60% susceptible to the juices. At 50% - 100% concentrations, C. albicans isolates were 100% sensitive to A. sativum extract. Conclusions: The medicinal plant juices tested against the pathogens possess some potentials worth exploiting as potent antimicrobial agents on gram-positive, gram-negative bacteria and the fungus.