Overexpression of the MYB37 transcription factor enhances abscisic acid sensitivity, and improves both drought tolerance and seed productivity in Arabidopsis thaliana.
Overexpression of the MYB37 transcription factor enhances abscisic acid sensitivity, and improves both drought tolerance and seed productivity in Arabidopsis thaliana.
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MYB37转录因子的过表达增强了拟南芥的脱落酸敏感性，并提高了耐旱性和种子生产力
DOI：
10.1007/s11103-015-0411-1
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发表时间：
2016-02
影响因子：
5.1
通讯作者：
Zhang DP
中科院分区：
文献类型：
--
作者：
Yu YT;Wu Z;Lu K;Bi C;Liang S;Wang XF;Zhang DP
虽然许多基因已经被揭示参与脱落酸（阿坝）信号传导，但许多参与阿坝信号传导的其他成分仍有待发现。我们报道了R2 R3 MYB亚组14转录因子MYB 37在拟南芥中的过表达，在所有主要的阿坝反应中，包括ABA诱导的种子萌发、子叶绿化和早期幼苗生长的抑制，以及ABA诱导的气孔关闭和气孔开放的抑制，都赋予了对外源阿坝过敏的表型。有趣且重要的是，MYB 37-过表达提高了植物对干旱的耐受性，增强了成熟植物的生长和种子生产力，但延迟了开花，这表明该基因可能用于提高作物对干旱环境的适应性和生产力。然而，amyb 37 - 1敲除突变体显示野生型阿坝反应最有可能是由于多个MYB成员的功能冗余。实时PCR分析显示MYB 37表达的上调改变了ABA应答基因的一个子集的表达。这些发现表明MYB 37转录因子在植物对阿坝和干旱胁迫的响应中起着重要的积极作用，同时，它在种子生产中起着积极的调节作用。
Although a lot of genes have been revealed to participate in abscisic acid (ABA) signaling, many of the additional components involved in ABA signaling remain to be discovered. Here we report that overexpression ofMYB37, a R2R3 MYB subgroup 14 transcription factor inArabidopsis thaliana, confers hypersensitive phenotypes to exogenous ABA in all the major ABA responses, including ABA-induced inhibition of seed germination, cotyledon greening and early seedling growth, and ABA-induced stomatal closure and inhibition of stomatal opening. Interestingly and importantly,MYB37-overexpression improves plant tolerance to drought, enhances growth of mature plants and seed productivity, thought it delays flowering, which suggests that this gene may be used for improving crop adaptability to drought environment and productivity. However, amyb37-1knockout mutant displays wild-type ABA responses most likely due to a functional redundancy of the multiple MYB members. Real-time PCR analysis shows that upregulation of theMYB37expression changes expression of a subset of ABA-responsive genes. Together, these findings suggest that the MYB37 transcription factor plays an important, positive role in plant response to ABA and drought stress, and meanwhile, it plays a positive role in the regulation of seed production.