Functional Imaging of the Outer Retinal Complex using High Fidelity Imaging Retinal Densitometry.
Functional Imaging of the Outer Retinal Complex using High Fidelity Imaging Retinal Densitometry.
复制标题
使用高保真成像视网膜密度测定法对外视网膜复合体进行功能成像。
DOI：
10.1038/s41598-020-60660-9
复制
发表时间：
2020
影响因子：
4.6
通讯作者：
Margrain TH
中科院分区：
文献类型：
--
作者：
Margrain TH
我们描述了一种新的技术，高保真成像视网膜密度测定（IRD），探测外部视网膜复合体的功能完整性。我们证明了该技术的能力，地图的视觉色素的光密度和合成率在眼睛和黄斑疾病。一种多光谱视网膜成像装置获得了视网膜反射率在空间和时间上的精确测量。从健康对照组和5例中度AMD患者中获得的数据，在色素漂白之前和之后，用于量化视觉色素指标。绘制热图以总结视杆细胞和视锥细胞色素动力学的地形图，并进行描述性统计以突出患有和不患有AMD的那些之间的差异。AMD患者的视杆细胞和视锥细胞色素合成率（分别为v= 0.043 SD 0.019 min− 1和v = 0.119 SD 0.046 min−1）约为健康对照组的一半（视杆细胞v= 0.079 SD 0.024 min− 1，视锥细胞v = 0.206 SD 0.069 min− 1）。通过绘制整个中央视网膜的视觉色素动力学，高保真IRD提供了对外部视网膜复合体功能的独特见解。这项新技术将改善包括AMD在内的各种眼部病变的表型表征、诊断和治疗监测。
We describe a new technique, high fidelity Imaging Retinal Densitometry (IRD), which probes the functional integrity of the outer retinal complex. We demonstrate the ability of the technique to map visual pigment optical density and synthesis rates in eyes with and without macular disease. A multispectral retinal imaging device obtained precise measurements of retinal reflectance over space and time. Data obtained from healthy controls and 5 patients with intermediate AMD, before and after photopigment bleaching, were used to quantify visual pigment metrics. Heat maps were plotted to summarise the topography of rod and cone pigment kinetics and descriptive statistics conducted to highlight differences between those with and without AMD. Rod and cone visual pigment synthesis rates in those with AMD (v= 0.043 SD 0.019 min−1andv= 0.119 SD 0.046 min−1, respectively) were approximately half those observed in healthy controls (v= 0.079 SD 0.024 min−1for rods and v = 0.206 SD 0.069 min−1for cones). By mapping visual pigment kinetics across the central retina, high fidelity IRD provides a unique insight into outer retinal complex function. This new technique will improve the phenotypic characterisation, diagnosis and treatment monitoring of various ocular pathologies, including AMD.