Cdc42 regulates cranial suture morphogenesis and ossification
Cdc42 regulates cranial suture morphogenesis and ossification
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DOI：
10.1016/j.bbrc.2019.02.106
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发表时间：
2019-04-30
影响因子：
3.1
通讯作者：
Kamijo, Ryutaro
中科院分区：
文献类型：
--
作者：
Aizawa, Ryo;Yamada, Atsushi;Kamijo, Ryutaro
Cdc 42（细胞分裂周期42）是属于Rho蛋白家族的广泛表达的小GTP酶。之前，我们产生了肢芽间充质特异性Cdc 42失活小鼠（Cdc 42条件性敲除小鼠; Cdc 42（fl/fl）; Prx 1-Cre），其表现出短肢和颅骨畸形，尽管与颅骨表型相关的机制尚不清楚。在本研究中，我们研究了Cdc 42在颅骨发育中的作用。我们的研究结果表明，Cdc 42的损失造成的缺陷膜内骨化在颅骨组织，这是相关的颅缝形态发生基因，包括印度刺猬（Ihh）和骨形态发生蛋白（BMP）的表达减少。这些研究结果表明，Cdc 42在颅骨成骨中起着至关重要的作用，并在颅骨发育过程中受到Ihh和BMP介导的信号转导的控制。(C)2019爱思唯尔公司All rights reserved.
Cdc42 (cell division cycle 42) is ubiquitously expressed small GTPases belonging to the Rho family of proteins. Previously, we generated limb bud mesenchyme-specific Cdc42 inactivated mice (Cdc42 conditional knockout mice; Cdc42(fl/fl); Prx1-Cre), which showed short limbs and cranial bone deformities, though the mechanism related to the cranium phenotype was unclear. In the present study, we investigated the role of Cdc42 in cranial bone development. Our results showed that loss of Cdc42 caused a defect of intramembranous ossification in cranial bone tissues which is related to decreased expressions of cranial suture morphogenesis genes, including Indian hedgehog (Ihh) and bone morphogenetic proteins (BMPs). These findings demonstrate that Cdc42 plays a crucial role in cranial osteogenesis, and is controlled by Ihh- and BMP-mediated signaling during cranium development. (C) 2019 Elsevier Inc. All rights reserved.