THE DETECTION AND MIDGUT INTRACELLULAR LOCATION OF RICKETTSIA SYMBIONT IN THE CAMELLIA APHID (APHIS AURANTII)
THE DETECTION AND MIDGUT INTRACELLULAR LOCATION OF RICKETTSIA SYMBIONT IN THE CAMELLIA APHID (APHIS AURANTII)
复制标题
茶蚜（Aphis aurantii）立克次体共生体的检测及中肠细胞内定位
DOI：
10.15666/aeer/1705_1220312212
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发表时间：
2019-01-01
影响因子：
0.7
通讯作者：
Huang, X. L.
中科院分区：
文献类型：
--
作者：
Deng, J.;Liu, Q.;Huang, X. L.
立克次体是节肢动物中普遍存在的次生共生细菌，在过去 20 年中人们逐渐认识到其在宿主的生物学中发挥着重要作用。之前的一些研究报道了立克次体在几种蚜虫中的存在，位于细胞外的次级细菌细胞、鞘细胞或蚜虫血淋巴中。在本研究中，我们发现立克次体共生体感染茶蚜Aphis aurantii，感染率相对较高（22%）。此外，基于16S rRNA基因的新一代测序，A. aurantii样本中立克次体的相对丰度甚至可以达到59.1%，超过了蚜虫的主要内共生体Buchnera。通过使用透射电子显微镜，我们还在 A. aurantii 的中肠上皮细胞中观察到密集的杆状立克次体共生体群体。我们的研究首次证实立克次体可以侵入A. aurantii的中肠上皮细胞，为了解立克次体在蚜虫中的位置和可能的感染途径提供了新的见解。
The Rickettsia, secondary symbiotic bacteria prevalent in arthropods, has been gradually recognized to play a significant role in the biology of their hosts in the past 20 years. A few previous studies have reported the occurrence of Rickettsia in several aphid species, located in the secondary bacteriocyte, sheath cell, or aphid hemolymph extracellularly. In this study, we found that Rickettsia symbiont infected camellia aphid, Aphis aurantii, with a relatively high infection rate (22%). Moreover, based on the next-generation sequencing of 16S rRNA genes, the relative abundance of Rickettsia from an A. aurantii sample could even reach 59.1%, which exceeded that of Buchnera, the primary endosymbiont of aphids. By using transmission electron microscopy, we also observed dense populations of rod-shaped Rickettsia symbionts in midgut epithelial cells of A. aurantii. Our study is the first to confirm that the Rickettsia can invade into midgut epithelial cells of A. aurantii, offering new insights into the location and possible infection route of Rickettsia in aphids.