EVIDENCE FOR INVOLVEMENT OF PENICILLIN-BINDING PROTEIN-3 IN MUREIN SYNTHESIS DURING SEPTATION BUT NOT DURING CELL ELONGATION
EVIDENCE FOR INVOLVEMENT OF PENICILLIN-BINDING PROTEIN-3 IN MUREIN SYNTHESIS DURING SEPTATION BUT NOT DURING CELL ELONGATION
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DOI：
10.1128/jb.145.1.333-340.1981
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
PARK, JT
中科院分区：
文献类型：
--
作者：
BOTTA, GA;PARK, JT
呋拉西林（1 μ g/ml）和哌拉西林（5 μ g/ml）特异性结合青霉素结合蛋白3（PBP-3），而不结合完整大肠杆菌细胞中的其它主要PBP。在两个温度敏感的分裂突变体中，研究了这种特异性结合PBP-3对伸长和同步分裂细胞胞壁素合成的影响。coliBUG 6和E. coliJE 10730，后者具有不耐热的PBP-3。通过将培养物从非允许温度（42 ℃）转移到非允许温度（42 ℃）诱导同步细胞分裂。C）至30度。C. [14 C]二氨基庚二酸掺入完整细胞的胞壁蛋白和[14 C] N-乙酰葡糖胺掺入乙醚透化的细胞的胞壁蛋白，在分隔细胞中平均抑制42%。在非允许温度下生长的细丝中，没有检测到抑制，并且经常检测到10-15%的胞壁素合成刺激。在上述实验中使用的浓度下，这2种药物在伸长和分隔的完整细胞和乙醚处理的细胞中仅与PBP-3结合。这些结果支持的假设，PBP-3的活动是专门需要的隔胞壁蛋白的合成。
Furazlocillin (1 .mu.g/ml) and piperacillin (5 .mu.g/ml) bound specifically to penicillin-binding protein 3 (PBP-3) and not to the other major PBPs in intact Escherichia coli cells. The effect of this specific binding to PBP-3 on murein synthesis of elongating and synchronously septating cells was investigated in 2 thermosensitive division mutants, E. coli BUG6 and E. coli JE10730, the latter possessing a thermolabile PBP-3. Synchronous cell division was induced by shifting the cultures from the nonpermissive temperature (42.degree. C) to 30.degree. C. [14C]Diaminopimelic acid incorporation into murein of intact cells and [14C]N-acetylglucosamine incorporation into murein of cells, permeabilized with ether, was inhibited by an average of 42% in septating cells. In filaments growing at the nonpermissive temperature, one detected no inhibition and, frequently, a 10-15% stimulation of murein synthesis. The 2 drugs, at concentrations used in the above experiments, bound exclusively to PBP-3 in elongating and septating intact cells and in ether-treated cells. These results support the hypothesis that PBP-3 activity is exclusively required for septal murein synthesis.