Impedimetric Detection of Histamine in Bowel Fluids Using Synthetic Receptors with pH-Optimized Binding Characteristics
Impedimetric Detection of Histamine in Bowel Fluids Using Synthetic Receptors with pH-Optimized Binding Characteristics
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DOI：
10.1021/ac3026288
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发表时间：
2013-02-05
影响因子：
7.4
通讯作者：
Wagner, Patrick
中科院分区：
文献类型：
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作者：
Peeters, Marloes;Troost, Freddy J.;Wagner, Patrick
组胺是一种生物胺，在各种生理系统的有效运作中不可或缺。在以前的工作中，分子印迹聚合物（MIP）为基础的传感器平台与阻抗读出，它可以快速，低成本地测定组胺浓度的缓冲溶液中的pH值7-9。（1，2）对于诊断应用，组胺应在较宽的pH范围内可检测到，因为它主要发生在弱酸性环境中。为了理解这种pH依赖的MIP传感器的响应，我们提出了一个统计结合分析模型。在这个模型中，我们预测的理论性能的MIP丙烯酸在所需的pH值范围内，并验证这些结果的实验紫外-可见光谱，微量重量分析，和阻抗谱。使用阻抗读出，特异性和选择性检测组胺在生理相关的纳摩尔浓度范围内是可能的，在中性和弱酸性磷酸盐缓冲液。最后，该传感器平台被用于分析几个测试人员的轻度酸性肠液样本的组胺浓度。我们表明，该传感器提供了可靠的数据，在相关的浓度制度，这是独立的酶联免疫吸附试验（ELISA）测试验证。
Histamine is a biogenic amine that is indispensable in the efficient functioning of various physiological systems. In previous work, a molecularly imprinted polymer (MIP) based sensor platform with impedimetric read-out was presented which could rapidly and at low cost determine histamine concentrations in buffer solutions within pH 7-9.(1,2) For diagnostic applications, histamine should be detectable in a wider pH range as it mostly occurs in mildly acidic environments. To understand this pH-dependent response of the MIP sensor, we propose a statistical binding analysis model. Within this model, we predict the theoretical performance of MIP based on acrylic acid in the required pH range and verify these results experimentally by UV-vis spectroscopy, microgravimetry, and impedance spectroscopy. Using impedimetric read-out, specific and selective detection of histamine in the physiologically relevant nanomolar concentration range is possible in neutral and mildly acidic phosphate buffer. Finally, this sensor platform was used to analyze the histamine concentration of mildly acidic bowel fluid samples of several test persons. We show that this sensor provides reliable data in the relevant concentration regime, which was validated independently by enzyme-linked immuno sorbent assay (ELISA) tests.