High embryo sac fertility and diversity of abnormal embryo sacs detected in autotetraploid indica/japonica hybrids in rice by whole-mount eosin B-staining confocal laser scanning microscopy
High embryo sac fertility and diversity of abnormal embryo sacs detected in autotetraploid indica/japonica hybrids in rice by whole-mount eosin B-staining confocal laser scanning microscopy
复制标题
整体伊红 B 染色共聚焦激光扫描显微镜检测水稻同源四倍体籼粳杂交种的高胚囊育性和异常胚囊多样性
DOI：
10.1111/j.1439-0523.2008.01555.x
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发表时间：
2009-04-01
期刊：
影响因子：
2
通讯作者：
Liu, X. D.
中科院分区：
文献类型：
--
作者：
Hu, C. Y.;Zeng, Y. X.;Liu, X. D.
用共聚焦显微镜伊红B染色技术(WE-CLSM)观察了24个典型的同源四倍体(2n=4x=48)水稻杂交组合的成熟胚囊，并与它们原来的二倍体杂交组合(2n=2x=24)进行了比较。24个杂交组合中有4个表现出80%的胚囊受精率，13个组合的胚囊受精率超过70%。24个同源四倍体杂交组合的胚囊育性平均值为68.08%，比二倍体组合提高了17.33%。与二倍体杂交种相比，79.17%的同源四倍体杂交种具有较高的胚囊育性。在同源四倍体的杂交水稻中，出现了多种异常胚囊。同源四倍体和二倍体杂种的胚囊异常相似，但频率不同。在同源四倍体杂种中发现了一些新的异常类型，如卵器增大。同源四倍体杂种的胚囊受精率和各种异常的频率因亲本的不同而不同。在不同生长季节，同源四倍体杂交种的平均结实率均高于其二倍体杂交种。
An eosin B staining procedure for use with confocal microscopy (WE-CLSM) was used to examine the mature embryo sacs in 24 typical autotetraploid (2n = 4x = 48) indica/japonica hybrids and to compare with their original diploid (2n = 2x = 24) indica/japonica hybrids in rice. Four of the 24 hybrids showed > 80% embryo sac fertility, and 13 hybrids over 70%. The average embryo sac fertility of the 24 autotetraploid indica/japonica hybrids was 68.08%, which was 17.33% higher than that in diploid hybrids. When compared with the diploid hybrids, 79.17% of the autotetraploid hybrids had higher embryo sac fertility. A diversity of abnormal embryo sacs occurred in autotetraploid indica/japonica hybrids. Embryo sac abnormalities were similar for both autotetraploid and diploid hybrid, but their frequencies were different. Some novel abnormal types were found in autotetraploid hybrids, such as enlarged egg apparatus. The embryo sac fertilities and frequencies of various abnormalities in autotetraploid hybrids varied with parental genotype and environment. The average seed set of the autotetraploid hybrids were higher than their diploid hybrids in different growing seasons.